LOCUS Exported 5576 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBbS6c-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5576) AUTHORS Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD TITLE BglBrick vectors and datasheets: A synthetic biology platform for gene expression. JOURNAL J Biol Eng. 2011 Sep 20;5:12. PUBMED 21933410 REFERENCE 2 (bases 1 to 5576) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5576 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(49..1131) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 1093..1112 /label=LacI-R /note="LacI, reverse primer" promoter complement(1132..1209) /gene="lacI" /label=lacI promoter primer_bind 1415..1434 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" protein_bind 1502..1518 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 1525..1541 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 1609..2286 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 2319..2390 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2406..2433 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(2964..3914) /codon_start=1 /gene="rep101" /product="RepA protein needed for replication with the pSC101 origin" /label=Rep101 /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" terminator 4676..4770 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(4791..5450) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 5365..5384 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(5451..5553) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" ORIGIN 1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct 61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc 301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt 361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg 781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc 1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca 1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg 1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca 1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag 1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga 1501 attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag cacatcagca 1561 ggacgcactg accgaattca aaagatcttt taagaaggag atatacatat ggcgagtagc 1621 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt 1681 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct 1741 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag 1801 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa 1861 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt 1921 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg 1981 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa 2041 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt 2101 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa 2161 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac 2221 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt 2281 gcttaaggat ccaaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt 2341 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 2401 acactggctc accttcgggt gggcctttct gcgtttatac ctagggtacg ggttttgctg 2461 cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca 2521 gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg 2581 gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt 2641 tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat 2701 gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat 2761 ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa 2821 gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct 2881 ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga 2941 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt 3001 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata cttactttgc 3061 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg 3121 tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt tgttggtatt 3181 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta 3241 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat 3301 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc 3361 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta 3421 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa 3481 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat 3541 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg 3601 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc 3661 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca 3721 ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt 3781 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc 3841 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca 3901 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga 3961 tgggctagtc aatgataatt actagtcctt ttcccgggtg atctgggtat ctgtaaattc 4021 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga 4081 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa 4141 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt 4201 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa 4261 aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt 4321 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct 4381 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc 4441 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc 4501 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt 4561 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca 4621 gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg cttggattct 4681 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg 4741 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg 4801 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca 4861 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta 4921 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa 4981 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac 5041 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt 5101 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc 5161 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc 5221 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc 5281 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga 5341 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga 5401 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc 5461 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta 5521 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca gatatc //