LOCUS Exported 4402 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBbA4c-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4402) AUTHORS Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD TITLE BglBrick vectors and datasheets: A synthetic biology platform for gene expression. JOURNAL J Biol Eng. 2011 Sep 20;5:12. PUBMED 21933410 REFERENCE 2 (bases 1 to 4402) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4402 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1857..2534 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 2567..2638 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2654..2681 /label=T7Te terminator /note="phage T7 early transcription terminator" primer_bind complement(2709..2726) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2843..3388) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(2923..2942) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 3502..3596 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(3617..4276) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 4191..4210 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(4277..4379) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" ORIGIN 1 gacgtctcag cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc 61 cgctgctgtt ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg 121 agcgggagtt ttcgccgact cgcgcgccag ttccggcagt agcagctgca aaaattgcgg 181 cgttaaatcc ggcgtcggtt ccacacttaa aaatagcgcc agtcgctcca tcatattgcg 241 cagttcacga atattgcccg gccagtcgta gtgcaccagc acggtttcgc ttgcctgtaa 301 tccctggcgt aatgcggcag aaaacggggt ggagagcgcc gccagagaca ctttcaaaaa 361 gctttccgcc agtggcagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg 421 caaaatactc agccgataaa acaggtcacg gcgaaactgc ccttgccgca tatcttcttc 481 cagattgcag tgagtggcgc taatgacccg cacatccacc ggaacaggct gatgcccgcc 541 gacgcgggtg acctcttttt cttccagcac ccgcagcagc cgggtctgca acggcagcgg 601 catttcgcca atctcatcga gaaacagcgt acctccgtgg gcgatttcaa acagcccggc 661 gcgaccgccg cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc 721 cagcagcgat tcggcaatcg ccccgcagtt gactgcaaca aacggatgcg actttttgcc 781 ctgtcgcgca tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt 841 gcccgtcccc gtttccccct caatcaacac cgctgcactg gagcgggcat acagcaaaat 901 agtctgccgt acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg 961 agttctcagg gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat 1021 atccagcgca tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt 1081 cattccggct tcttctgcca gatcggtaat cagccccgcg ccgaccaccg cttcggtgcc 1141 gttagctttt agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt agctacgttg 1201 atcgaggcgc aaattaaagg ttttttgaaa cgccaccagt gccggaatag tttcctgata 1261 agtgacaacg ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc 1321 gtagccgctc ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc 1381 gttagatcca gcggcgatga tggcgtcaca gcgttcgttt gccagtttct tgtggatgta 1441 ggtcaccgct ttttcaaagc caagctggat aggggtaatg ttcgccaggt gatcaaactc 1501 aaggctgata tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg 1561 tttgtcgtca ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa 1621 tattgggttt agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa 1681 acgttaactg aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa 1741 ttaaacattt aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg 1801 caaataacaa gttgataaca agaattcaaa agatctttta agaaggagat atacatatgg 1861 cgagtagcga agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg 1921 ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc 1981 agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt 2041 ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact 2101 acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg 2161 gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag 2221 ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg 2281 gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca 2341 aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca 2401 tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca 2461 cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact 2521 ccaccggtgc ttaaggatcc aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa 2581 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2641 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct agggatatat 2701 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg 2761 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg 2821 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa 2881 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc 2941 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc 3001 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt 3061 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc 3121 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc 3181 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga 3241 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt 3301 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga 3361 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt 3421 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata 3481 taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt 3541 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag 3601 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat 3661 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg 3721 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga 3781 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg 3841 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac 3901 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc 3961 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat 4021 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca 4081 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct 4141 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact 4201 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag 4261 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata 4321 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa 4381 cgtctcattt tcgccagata tc //