LOCUS       Exported                 801 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    fos-tTA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Reijmers LG, Perkins BL, Matsuo N, Mayford M
  TITLE     Localization of a stable neural correlate of associative memory.
  JOURNAL   Science. 2007 Aug 31;317(5842):1230-3.
  PUBMED    17761885
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(274..294)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          424..489
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        573..591
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     573..590
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(661..687)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc
      121 actatacatc aaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt
      181 ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact
      241 tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt
      301 agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa
      361 caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg
      421 aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt
      481 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
      541 gaagtaaggt tccttcacaa agatcctcta gcatttaggt gacactatag aatagggccc
      601 tctagatgca tgctcgagcg gccggccgcc agtgtgatgg atggataatt cttagtcgag
      661 tgcgtagtct ggtacgtcgt acggatagtc gagatcctta tcacagcatg tccaggtcga
      721 agtcgtccag ggcgtcggcc ggcagcatgt ccaggtcgaa gtcgtccagg gcgtcggccg
      781 gcagcatgtc caggtcgaag t
//
