LOCUS Exported 5527 bp ds-DNA circular SYN 23-SEP-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pmCherry-ATG5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5527) AUTHORS Hamacher-Brady A, Brady NR, Logue SE, Sayen MR, Jinno M, Kirshenbaum LA, Gottlieb RA, Gustafsson AB TITLE Response to myocardial ischemia/reperfusion injury involves Bnip3 and autophagy. JOURNAL Cell Death Differ. 2006 Apr 28. ():. PUBMED 16645637 REFERENCE 2 (bases 1 to 5527) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 23, 2022 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5527 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 24..43 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 1318..1439 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1355..1374) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1409..1428 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1446..1901) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1583..1604) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1795..1814 /label=F1ori-R /note="F1 origin, reverse primer" promoter 1928..2032 /gene="bla" /label=AmpR promoter promoter 2034..2392 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2057..2077) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2243..2378 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2305..2324 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2427..3221 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2481..2500) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3091..3110 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3409..3428) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3453..3500 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3829..4417 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4318..4337 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 4592..4895 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4896..5099 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5050..5070 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 5138..318 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(5283..5301) /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 61 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 121 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 181 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 241 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 301 atggacgagc tgtacaagag atctcgagct caagcttcga attctgcagt cgacggtacc 361 atgacagatg acaaagatgt gcttcgagat gtgtggtttg gacgaattcc aacttgcttt 421 actctctatc aggatgagat aactgaaaga gaagcagaac catactattt gcttttgcca 481 agagtcagct atttgacgtt ggtaactgac aaagtgaaaa agcactttca gaaggttatg 541 agacaagaag atgttagtga gatatggttt gaatatgaag gcacacccct gaaatggcat 601 tatccaattg gtttactatt tgatcttctt gcatcaagtt cagctcttcc ttggaacatc 661 acagtacatt tcaagagttt tccagaaaag gaccttctac actgtccatc caaggatgcg 721 gttgaggctc actttatgtc gtgtatgaaa gaagctgatg ctttaaagca taaaagtcaa 781 gtgatcaacg aaatgcagaa aaaagaccac aagcagctct ggatgggact gcagaatgac 841 agatttgacc agttttgggc catcaaccgg aaactcatgg aatatcctcc agaagaaaat 901 ggatttcgtt atatcccctt tagaatatat cagaccacga cggagcggcc tttcatccag 961 aagctgttcc ggcctgtggc cgcagatgga cagctgcaca cacttggaga tctcctcaga 1021 gaagtctgtc cttccgcagt cgcccctgaa gatggagaga agaggagcca ggtgatgatt 1081 cacgggatag agccaatgct ggaaacccct ctgcagtggc tgagcgagca tctgagctac 1141 ccagataact ttcttcatat tagcattgtc ccccagccaa cagattgagg atccaccgga 1201 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 1261 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 1321 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 1381 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 1441 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 1501 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 1561 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 1621 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 1681 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 1741 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 1801 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 1861 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 1921 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 1981 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 2041 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 2101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 2161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaatttagt cagcaaccat 2221 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 2281 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 2341 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 2401 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 2461 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 2521 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 2581 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 2641 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 2701 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 2761 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 2821 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 2881 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 2941 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 3001 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 3061 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 3121 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 3181 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 3241 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 3301 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 3361 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 3421 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 3481 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 3541 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 3601 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 3661 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 3721 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 3781 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 3841 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 3901 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 3961 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 4021 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 4081 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 4141 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 4201 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 4261 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 4321 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 4381 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 4441 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 4501 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt 4561 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 4621 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 4681 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 4741 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 4801 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 4861 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 4921 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 4981 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 5041 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg 5101 gtttagtgaa ccgtcagatc cgctagcgct accggtaatg gtgagcaagg gcgaggagga 5161 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 5221 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 5281 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 5341 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 5401 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 5461 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa 5521 gctgcgc //