LOCUS Exported 13118 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBbA5c-MevT(CO)-MBIS(CO, ispA) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13118) AUTHORS Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS TITLE Identification and microbial production of a terpene-based advanced biofuel. JOURNAL Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. PUBMED 21952217 REFERENCE 2 (bases 1 to 13118) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13118 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(49..1131) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 1093..1112 /label=LacI-R /note="LacI, reverse primer" promoter complement(1132..1209) /gene="lacI" /label=lacI promoter primer_bind 1415..1434 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" promoter 1520..1550 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind 1558..1574 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 5220..5231 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 8840..8851 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" terminator 11283..11354 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 11370..11397 /label=T7Te terminator /note="phage T7 early transcription terminator" primer_bind complement(11425..11442) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11559..12104) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(11639..11658) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 12218..12312 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(12333..12992) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 12907..12926 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(12993..13095) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" ORIGIN 1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct 61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc 301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt 361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg 781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc 1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca 1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg 1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca 1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag 1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga 1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg 1561 tgagcggata acaatttcag aattcaaaag atctaaagga ggccatcctg gccatgaaga 1621 actgtgtgat tgtttctgcg gtccgcacgg cgatcggcag ctttaacggc tctttagcga 1681 gcacctctgc aatcgatctg ggtgcgacgg tcattaaggc cgccattgaa cgcgccaaaa 1741 tcgacagcca gcacgttgat gaggtgatca tgggcaatgt gttacaagcc ggcctgggtc 1801 aaaacccagc gcgtcaagca ctgttaaaat ctggtctggc cgagaccgtg tgtggcttca 1861 ccgtcaataa ggtttgcggc tctggcctga agagcgtggc cctggcagca caagcgattc 1921 aagccggtca ggcacaaagc atcgttgcgg gtggcatgga gaacatgtct ctggcgccgt 1981 acttattaga tgccaaagcc cgcagcggtt atcgcctggg cgatggtcag gtgtacgacg 2041 tcatcttacg cgatggctta atgtgcgcga cccacggtta ccacatgggt attacggccg 2101 aaaacgtggc gaaagaatac ggcattacgc gcgagatgca ggatgaatta gcactgcact 2161 ctcagcgcaa agcagcagcc gcgatcgagt ctggtgcgtt tacggcggaa atcgtgccag 2221 ttaacgtggt cacgcgcaag aagacgttcg ttttcagcca ggacgagttc ccgaaggcaa 2281 acagcaccgc ggaggcctta ggtgccttac gcccagcctt tgacaaagcg ggcacggtca 2341 ccgccggtaa tgcgagcggc atcaatgatg gtgcagcggc actggtcatc atggaagaga 2401 gcgccgcatt agcagcgggt ctgaccccat tagcgcgcat taaatcttat gccagcggcg 2461 gcgtcccacc agccctgatg ggcatgggtc cggtcccagc cacgcaaaaa gccctgcaat 2521 tagcgggcct gcaactggcc gacattgatc tgatcgaggc gaacgaggcg tttgcagcgc 2581 agttcctggc ggtgggtaag aatctgggct tcgacagcga gaaagtcaat gtgaacggtg 2641 gcgcgattgc gttaggccat ccgattggtg caagcggcgc acgcatctta gtgacgttac 2701 tgcacgccat gcaggcacgc gacaagacct taggcctggc gaccttatgt attggtggcg 2761 gtcaaggtat cgccatggtg atcgaacgcc tgaactgatg aaggaggaaa gcaaaatgaa 2821 actgagcacc aagctgtgct ggtgtggcat caagggtcgc ctgcgcccac aaaagcagca 2881 acagctgcac aacacgaacc tgcaaatgac cgagctgaaa aagcagaaga cggccgagca 2941 aaagacccgc ccgcagaacg ttggcatcaa gggcatccag atttatatcc cgacgcagtg 3001 tgtcaaccaa tctgagctgg agaaattcga tggcgtcagc cagggtaagt acaccatcgg 3061 cctgggccag accaacatga gcttcgtgaa cgaccgtgag gacatctatt ctatgagcct 3121 gacggtgctg tctaagctga tcaagagcta caacatcgac acgaataaga tcggtcgtct 3181 ggaggtgggt acggagacgc tgattgacaa gagcaaaagc gtgaagtctg tcttaatgca 3241 gctgttcggc gagaacacgg atgtcgaggg tatcgacacc ctgaacgcgt gttacggcgg 3301 caccaacgca ctgttcaata gcctgaactg gattgagagc aacgcctggg atggccgcga 3361 tgcgatcgtc gtgtgcggcg atatcgccat ctatgacaag ggtgcggcac gtccgaccgg 3421 cggtgcaggc accgttgcga tgtggattgg cccggacgca ccaattgtct tcgattctgt 3481 ccgcgcgtct tacatggagc acgcctacga cttttacaag ccggacttca cgagcgaata 3541 cccgtacgtg gacggccact tctctctgac ctgctatgtg aaggcgctgg accaggttta 3601 taagtcttat agcaaaaagg cgatttctaa gggcctggtc agcgacccgg caggcagcga 3661 cgccctgaac gtgctgaagt atttcgacta caacgtgttc catgtcccga cctgcaaatt 3721 agtgaccaaa tcttatggcc gcctgttata taatgatttc cgtgccaacc cgcagctgtt 3781 cccggaggtt gacgccgagc tggcgacgcg tgattacgac gagagcctga ccgacaagaa 3841 catcgagaag accttcgtca acgtcgcgaa gccgttccac aaagagcgtg tggcccaaag 3901 cctgatcgtc ccgaccaaca cgggcaacat gtataccgcg tctgtctacg cggcattcgc 3961 gagcctgctg aattacgtcg gttctgacga cctgcagggc aagcgcgttg gcctgttcag 4021 ctacggtagc ggcttagcgg ccagcctgta tagctgcaaa attgtcggcg acgtccagca 4081 catcatcaag gagctggaca tcaccaacaa gctggcgaag cgcatcaccg agacgccgaa 4141 agattacgag gcagcgatcg agttacgcga gaatgcgcat ctgaagaaga acttcaagcc 4201 gcaaggtagc atcgagcacc tgcagagcgg cgtctactac ctgacgaaca ttgacgacaa 4261 gttccgccgt tcttatgacg tcaaaaagta actagtagga ggaaaacatc atggtgctga 4321 cgaacaaaac cgtcattagc ggcagcaagg tgaagtctct gagcagcgcc caaagctcta 4381 gcagcggccc gtctagcagc agcgaggagg acgacagccg tgacattgag tctctggaca 4441 agaagatccg cccgctggag gagttagagg ccctgctgag cagcggcaac accaagcagc 4501 tgaagaacaa ggaagttgca gcgctggtga tccacggtaa gctgccactg tatgcgctgg 4561 aaaagaaact gggcgatacg acgcgtgcgg tcgcggtgcg tcgcaaagcc ttaagcatct 4621 tagcggaggc cccggtgtta gccagcgacc gcctgccgta caagaactac gactacgacc 4681 gcgtgtttgg cgcgtgctgc gagaatgtca ttggctacat gccgttaccg gttggtgtga 4741 tcggcccgct ggtcattgat ggcacgagct atcacattcc aatggcgacc acggaaggtt 4801 gcttagtcgc cagcgccatg cgtggctgta aggcgattaa cgccggcggt ggcgcgacga 4861 ccgtgttaac caaggatggt atgacgcgcg gtccggtcgt ccgcttccca acgctgaagc 4921 gcagcggcgc gtgtaagatt tggctggatt ctgaggaggg ccaaaacgcg atcaagaaag 4981 ccttcaactc tacgagccgt ttcgcgcgtt tacagcatat ccagacctgc ctggccggcg 5041 acctgctgtt catgcgcttc cgcaccacca cgggcgatgc gatgggcatg aacatgatca 5101 gcaagggcgt cgaatatagc ctgaaacaaa tggtggaaga atatggctgg gaggacatgg 5161 aggttgtctc tgtgagcggc aactattgca ccgacaagaa gccggcagcc attaactgga 5221 ttgagggtcg cggcaaaagc gtcgtggcag aagcgaccat cccaggcgac gtggtccgta 5281 aggttctgaa gagcgacgtc agcgccctgg ttgagttaaa tatcgcgaaa aacctggtcg 5341 gcagcgcgat ggcgggcagc gtgggtggct ttaacgcaca tgcagcgaat ctggttacgg 5401 cggttttctt agccttaggt caggacccag cccaaaatgt cgagagcagc aactgcatta 5461 ccttaatgaa agaggttgac ggtgacctgc gcatcagcgt ttctatgccg tctatcgagg 5521 tcggcacgat cggcggcggc accgttttag aaccgcaagg tgcgatgctg gatctgctgg 5581 gcgtgcgcgg cccacatgca acggccccag gcaccaatgc ccgccaactg gcccgtatcg 5641 tggcctgcgc ggttctggcg ggtgagctga gcctgtgcgc cgcattagcc gcgggccatt 5701 tagttcaatc tcacatgacc cacaaccgca agccggcaga accaaccaag ccaaataacc 5761 tggacgcaac cgacattaac cgtctgaagg atggcagcgt cacgtgcatt aaaagctgat 5821 gaggatctag gaggaaataa ccatgtctct gccattcctg acgtctgcgc caggtaaggt 5881 gatcatcttc ggcgagcact ctgcggtgta caataagccg gccgtcgccg cctctgtgtc 5941 tgcgttacgc acctacctgc tgatcagcga atcttctgca ccggacacga tcgagctgga 6001 ctttccggac atcagcttca accacaagtg gagcatcaac gacttcaacg cgatcacgga 6061 ggaccaggtg aacagccaaa agctggccaa agcccagcaa gcaaccgacg gtctgtctca 6121 ggagctggtg tctctgctgg acccgctgtt agcgcagtta agcgagagct tccattacca 6181 cgccgcgttc tgcttcctgt acatgttcgt ttgcctgtgc ccgcacgcaa agaacatcaa 6241 gttcagcctg aagagcacgc tgccgattgg cgcaggctta ggctctagcg catctatcag 6301 cgtgagcctg gcgctggcga tggcctatct gggtggcctg attggcagca acgacctgga 6361 gaaactgagc gaaaacgaca agcacatcgt gaaccagtgg gcctttatcg gcgagaagtg 6421 cattcatggc accccgagcg gcattgacaa cgcagttgcc acgtatggca acgccctgct 6481 gttcgagaaa gacagccaca acggcacgat caacacgaac aacttcaagt tcctggacga 6541 cttcccggcg atcccgatga ttctgaccta cacccgtatc ccacgcagca ccaaggattt 6601 agtcgcccgc gtgcgtgttt tagtcaccga aaagttcccg gaggtgatga agccgatcct 6661 ggacgcgatg ggcgagtgcg cgctgcaggg tctggagatc atgaccaagc tgagcaagtg 6721 caagggcacc gacgatgagg cggtggagac caacaatgag ctgtacgagc agctgctgga 6781 gctgatccgt atcaatcacg gcctgctggt ctctatcggt gtgtctcacc cgggcctgga 6841 actgatcaaa aacctgagcg acgacctgcg cattggctct acgaaattaa cgggtgcagg 6901 tggcggtggc tgctctttaa cgctgctgcg ccgtgacatt acgcaggagc aaatcgacag 6961 cttcaagaag aagctgcagg acgacttcag ctacgagacg ttcgagacgg acctgggcgg 7021 cacgggctgt tgcctgctga gcgccaaaaa tctgaacaag gacctgaaga tcaaaagcct 7081 ggtgttccag ctgttcgaaa acaagacgac cacgaagcag cagatcgacg acctgttact 7141 gccgggtaac accaatctgc cgtggacgtc ttaaggatct aggagggaga tcatatgagc 7201 gaattacgtg cattcagcgc gccaggtaag gcactgctgg ccggtggcta cctggtgtta 7261 gacaccaagt acgaggcgtt cgtcgtcggc ttatctgccc gtatgcatgc agttgcccac 7321 ccgtatggta gcctgcaggg ctctgacaag ttcgaagtgc gtgtgaagag caagcagttc 7381 aaggacggcg agtggctgta ccacattagc ccaaagagcg gcttcatccc ggttagcatt 7441 ggtggcagca agaacccatt tatcgagaag gtcattgcca acgtcttcag ctacttcaag 7501 ccgaatatgg acgattactg caaccgcaac ctgttcgtca tcgacatttt cagcgacgac 7561 gcgtaccaca gccaagagga ctctgttacg gagcatcgtg gtaaccgccg cctgagcttc 7621 cacagccatc gcattgagga ggtgccgaag acgggtctgg gttctagcgc cggtttagtt 7681 accgtcttaa cgacggcgtt agcgagcttc ttcgtgagcg acctggagaa caacgtggac 7741 aagtaccgcg aagtgattca taacctggcg caggtggcac attgtcaggc ccaaggtaag 7801 attggctctg gttttgatgt ggcagcggcc gcctatggct ctatccgcta tcgccgcttt 7861 ccgccggccc tgatcagcaa tctgccggac atcggctctg cgacgtatgg tagcaaactg 7921 gcgcatctgg tggacgagga ggactggaac atcaccatta agtctaatca cctgccgagc 7981 ggcttaacgt tatggatggg cgatatcaag aacggcagcg aaacggttaa gctggtgcag 8041 aaagtgaaaa actggtacga cagccacatg ccggaaagcc tgaagattta cacggagctg 8101 gaccacgcca atagccgttt catggatggt ctgagcaagc tggaccgcct gcacgaaacc 8161 cacgacgact acagcgacca aatcttcgag agcctggagc gcaatgactg cacctgccag 8221 aagtacccgg agatcacgga ggtccgcgat gccgtggcaa cgattcgccg tagcttccgc 8281 aaaattacga aggagagcgg cgcggatatc gaaccaccgg tccagacgtc tctgctggac 8341 gactgtcaaa ccttaaaggg cgtgttaacg tgcctgattc cgggcgcggg tggttacgac 8401 gccattgccg tcatcacgaa acaggacgtc gatctgcgcg cacaaacggc caacgacaaa 8461 cgtttcagca aagtccaatg gctggatgtt acgcaggccg actggggtgt tcgcaaggag 8521 aaggacccgg aaacgtatct ggataagtga ggatctagga ggattatgag atgaccgttt 8581 acacagcatc cgttaccgca cccgtcaaca tcgcaaccct taagtattgg gggaaaaggg 8641 acacgaagtt gaatctgccc accaattcgt ccatatcagt gactttatcg caagatgacc 8701 tcagaacgtt gacctctgcg gctactgcac ctgagtttga acgcgacact ttgtggttaa 8761 atggagaacc acacagcatc gacaatgaaa gaactcaaaa ttgtctgcgc gacctacgcc 8821 aattaagaaa ggaaatggaa tcgaaggacg cctcattgcc cacattatct caatggaaac 8881 tccacattgt ctccgaaaat aactttccta cagcagctgg tttagcttcc tccgctgctg 8941 gctttgctgc attggtctct gcaattgcta agttatacca attaccacag tcaacttcag 9001 aaatatctag aatagcaaga aaggggtctg gttcagcttg tagatcgttg tttggcggat 9061 acgtggcctg ggaaatggga aaagctgaag atggtcatga ttccatggca gtacaaatcg 9121 cagacagctc tgactggcct cagatgaaag cttgtgtcct agttgtcagc gatattaaaa 9181 aggatgtgag ttccactcag ggtatgcaat tgaccgtggc aacctccgaa ctatttaaag 9241 aaagaattga acatgtcgta ccaaagagat ttgaagtcat gcgtaaagcc attgttgaaa 9301 aagatttcgc cacctttgca aaggaaacaa tgatggattc caactctttc catgccacat 9361 gtttggactc tttccctcca atattctaca tgaatgacac ttccaagcgt atcatcagtt 9421 ggtgccacac cattaatcag ttttacggag aaacaatcgt tgcatacacg tttgatgcag 9481 gtccaaatgc tgtgttgtac tacttagctg aaaatgagtc gaaactcttt gcatttatct 9541 ataaattgtt tggctctgtt cctggatggg acaagaaatt tactactgag cagcttgagg 9601 ctttcaacca tcaatttgaa tcatctaact ttactgcacg tgaattggat cttgagttgc 9661 aaaaggatgt tgccagagtg attttaactc aagtcggttc aggcccacaa gaaacaaacg 9721 aatctttgat tgacgcaaag actggtctac caaaggaata aggatctagg aggtaatgat 9781 aatgcaaacg gaacacgtca ttttattgaa tgcacaggga gttcccacgg gtacgctgga 9841 aaagtatgcc gcacacacgg cagacacccg cttacatctc gcgttctcca gttggctgtt 9901 taatgccaaa ggacaattat tagttacccg ccgcgcactg agcaaaaaag catggcctgg 9961 cgtgtggact aactcggttt gtgggcaccc acaactggga gaaagcaacg aagacgcagt 10021 gatccgccgt tgccgttatg agcttggcgt ggaaattacg cctcctgaat ctatctatcc 10081 tgactttcgc taccgcgcca ccgatccgag tggcattgtg gaaaatgaag tgtgtccggt 10141 atttgccgca cgcaccacta gtgcgttaca gatcaatgat gatgaagtga tggattatca 10201 atggtgtgat ttagcagatg tattacacgg tattgatgcc acgccgtggg cgttcagtcc 10261 gtggatggtg atgcaggcga caaatcgcga agccagaaaa cgattatctg catttaccca 10321 gcttaaataa ggatctagga ggataaagaa atggactttc cgcagcaact cgaagcctgc 10381 gttaagcagg ccaaccaggc gctgagccgt tttatcgccc cactgccctt tcagaacact 10441 cccgtggtcg aaaccatgca gtatggcgca ttattaggtg gtaagcgcct gcgacctttc 10501 ctggtttatg ccaccggtca tatgttcggc gttagcacaa acacgctgga cgcacccgct 10561 gccgccgttg agtgtatcca cgcttactca ttaattcatg atgatttacc ggcaatggat 10621 gatgacgatc tgcgtcgcgg tttgccaacc tgccatgtga agtttggcga agcaaacgcg 10681 attctcgctg gcgacgcttt acaaacgctg gcgttctcga ttttaagcga tgccgatatg 10741 ccggaagtgt cggaccgcga cagaatttcg atgatttctg aactggcgag cgccagtggt 10801 attgccggaa tgtgcggtgg tcaggcatta gatttagacg cggaaggcaa acacgtacct 10861 ctggacgcgc ttgagcgtat tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt 10921 cgccttggtg cattaagcgc cggagataaa ggacgtcgtg ctctgccggt actcgacaag 10981 tatgcagaga gcatcggcct tgccttccag gttcaggatg acatcctgga tgtggtggga 11041 gatactgcaa cgttgggaaa acgccagggt gccgaccagc aacttggtaa aagtacctac 11101 cctgcacttc tgggccttga gcaagcccgg aagaaagccc gggatctgat cgacgatgcc 11161 cgtcagtcgc tgaaacaact ggctgaacag tcactcgata cctcggcact ggaagcgcta 11221 gcggactaca tcatccagcg taataaataa ggatccaaac tcgagtaagg atctccaggc 11281 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 11341 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 11401 atacctaggg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact 11461 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat 11521 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct 11581 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa 11641 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg 11701 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag 11761 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga 11821 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc 11881 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg 11941 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc 12001 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt 12061 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta 12121 atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag 12181 tcagccccat acgatataag ttgttactag tgcttggatt ctcaccaata aaaaacgccc 12241 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta 12301 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag 12361 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga 12421 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg 12481 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc 12541 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc 12601 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg 12661 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa 12721 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc 12781 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta 12841 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta 12901 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca 12961 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc 13021 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct 13081 cttacgtgcc gatcaacgtc tcattttcgc cagatatc //