LOCUS Exported 12735 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS TALE11 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12735) AUTHORS Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P TITLE Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. JOURNAL Nat Biotechnol. 2011 Jan 19. ():. PUBMED 21248753 REFERENCE 2 (bases 1 to 12735) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12735 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2682..3859 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2913..3850 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3807..3827 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 6416..6436 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6461..6610 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 6632..6685 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6686..7402 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 6686..7399 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(6728..6749) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6989..7008) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7336..7357 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7427..8015 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7480..7500) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7898..7909) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(8018..8034) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8019..8035) /label=pBluescriptKS /note="For pBluescript vector" LTR 8540..8720 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8746..8763) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8752..8976 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9022..9450 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9109..9128) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9319..9340 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9459..9479) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9464..9793 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9644..9779 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9706..9725 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9841..9888 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9907..10281 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 10411..10532 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10448..10467) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10502..10521 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10581..10597) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10581..10597) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10594..10616) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10605..10621 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10629..10659) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10674..10695 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10812..10829) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10983..11571) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11063..11082) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11742..12602) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12365..12384 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12603..12707) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt 2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt 2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta 3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc 3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg 3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat 3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc 3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg 3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg 3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat 3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg 3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg 3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg 3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag 3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg 3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct 3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct 3841 tccatttcag gtgtcgtgac gtacggccac catgtcgcgg acccggctcc cttccccacc 3901 cgcacccagc ccagcgtttt cggccgactc gttctcagac ctgcttaggc agttcgaccc 3961 ctcactgttt aacacatcgt tgttcgactc ccttcctccg tttggggcgc accatacgga 4021 ggcggccacc ggggagtggg atgaggtgca gtcgggattg agagctgcgg atgcaccacc 4081 cccaaccatg cgggtggccg tcaccgctgc ccgaccgccg agggcgaagc ccgcaccaag 4141 gcggagggca gcgcaaccgt ccgacgcaag ccccgcagcg caagtagatt tgagaacttt 4201 gggatattca cagcagcagc aggaaaagat caagcccaaa gtgaggtcga cagtcgcgca 4261 gcatcacgaa gcgctggtgg gtcatgggtt tacacatgcc cacatcgtag ccttgtcgca 4321 gcaccctgca gcccttggca cggtcgccgt caagtaccag gacatgattg cggcgttgcc 4381 ggaagccaca catgaggcga tcgtcggtgt ggggaaacag tggagcggag cccgagcgct 4441 tgaggccctg ttgacggtcg cgggagagct gagagggcct ccccttcagc tggacacggg 4501 ccagttgctg aagatcgcga agcggggagg agtcacggcg gtcgaggcgg tgcacgcgtg 4561 gcgcaatgcg ctcacgggag cacccctcaa cctgacccca gagcaggtcg tggcgatcgc 4621 aagccacgac ggaggaaagc aagccttgga aacagtacag aggctgttgc ctgtgctgtg 4681 ccaagcgcac ggactcaccc cagagcaggt cgtggccatt gcctcgaatg gagggggcaa 4741 acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acggcctcac 4801 cccagagcag gtcgtggcga tcgcaagcca cgacggagga aagcaagcct tggaaacagt 4861 acagaggctg ttgcctgtgc tgtgccaagc gcacggatta accccagagc aggtcgtggc 4921 cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat tgctgccggt 4981 gctgtgccaa gcgcacggct taaccccaga gcaggtcgtg gccattgcct cgaatggagg 5041 gggcaaacag gcgttggaaa ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg 5101 acttacccca gagcaggtcg tggcgatcgc aagccacgac ggaggaaagc aagccttgga 5161 aacagtacag aggctgttgc ctgtgctgtg ccaagcgcac ggccttaccc cagagcaggt 5221 cgtggcgatc gcaagccacg acggaggaaa gcaagccttg gaaacagtac agaggctgtt 5281 gcctgtgctg tgccaagcgc acggactaac cccagagcag gtcgtggcga tcgcaagcca 5341 cgacggagga aagcaagcct tggaaacagt acagaggctg ttgcctgtgc tgtgccaagc 5401 gcacgggctc accccagagc aggtcgtggc cattgcctcg aatggagggg gcaaacaggc 5461 gttggaaacc gtacaacgat tgctgccggt gctgtgccaa gcgcacgggc taaccccaga 5521 gcaggtcgtg gcgatcgcaa gccacgacgg aggaaagcaa gccttggaaa cagtacagag 5581 gctgttgcct gtgctgtgcc aagcgcacgg cctaacccca gagcaggtcg tggcgatcgc 5641 aagccacgac ggaggaaagc aagccttgga aacagtacag aggctgttgc ctgtgctgtg 5701 ccaagcgcac gggttaaccc cagagcaggt cgtggccatt gcctcgaatg gagggggcaa 5761 acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acggactcac 5821 gcctgagcag gtagtggcta ttgcatccaa tggcgggggc agacccgcac tggagtcaat 5881 cgtggcccag ctttcgaggc cggaccccgc gctggccgca ctcactaatg atcatcttgt 5941 agcgctggcc tgcctcggcg gacgacccgc cttggatgcg gtgaagaagg ggctcccgca 6001 cgcgcctgca ttgattaagc ggaccaacag aaggattccc gagaggacat cacatcgagt 6061 ggcagatcac gcgcaagtgg tccgcgtgct cggattcttc cagtgtcact cccaccccgc 6121 acaagcgttc gatgacgcca tgactcaatt tggtatgtcg agacacggac tgctgcagct 6181 ctttcgtaga gtcggtgtca cagaactcga ggcccgctcg ggcacactgc ctcccgcctc 6241 ccagcggtgg gacaggattc tccaagcgag cggtatgaaa cgcgcgaagc cttcacctac 6301 gtcaactcag acacctgacc aggcgagcct tcatgcgttc gcagactcgc tggagaggga 6361 tttggacgcg ccctcgccca tgcatgaagg ggaccaaact cgcgcgtcag ctagccccaa 6421 gaagaagaga aaggtggagg ccagcggttc cggacgggct gacgcattgg acgattttga 6481 tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga 6541 tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct 6601 ggacatgctg attaactcta gaggcagtgg agagggcaga ggaagtctgc taacatgcgg 6661 tgacgtcgag gagaatcctg gcccagtgag caagggcgag gagctgttca ccggggtggt 6721 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 6781 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 6841 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 6901 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 6961 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 7021 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 7081 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 7141 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 7201 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 7261 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 7321 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 7381 catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc aacctctgga 7441 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 7501 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 7561 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 7621 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 7681 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 7741 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 7801 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 7861 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 7921 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 7981 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct 8041 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg 8101 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag 8161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 8221 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac 8281 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact 8341 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa 8401 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga 8461 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga 8521 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 8581 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 8641 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 8701 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt 8761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 8821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 8881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 8941 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 9001 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 9061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 9121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 9181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 9241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 9301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 9361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 9421 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 9481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 9541 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 9601 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 9661 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 9721 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 9781 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt 9841 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac 9901 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga 9961 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc 10021 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg 10081 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg 10141 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc 10201 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc 10261 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat 10321 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 10381 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 10441 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 10501 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 10561 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 10621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 10681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 10741 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 10801 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 10861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 10921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 10981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 11041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 11101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 11161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 11221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 11281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 11341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 11401 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 11461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 11521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 11581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 11641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 11701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 11761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 11821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 11881 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 11941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 12001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 12061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 12121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 12181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 12241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 12301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 12361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 12421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 12481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 12541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 12601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 12661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 12721 aaagtgccac ctgac //