LOCUS Exported 11082 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-C1C2-TS-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11082) AUTHORS Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, Ishitani R, Deisseroth K, Nureki O TITLE Crystal structure of the channelrhodopsin light-gated cation channel. JOURNAL Nature. 2012 Jan 22. doi: 10.1038/nature10870. PUBMED 22266941 REFERENCE 2 (bases 1 to 11082) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11082 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3890 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" CDS 5033..5749 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(5075..5096) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5336..5355) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5683..5704 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5774..6362 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5827..5847) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6245..6256) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6365..6381) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6366..6382) /label=pBluescriptKS /note="For pBluescript vector" LTR 6887..7067 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7093..7110) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7099..7323 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7369..7797 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7456..7475) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7666..7687 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7806..7826) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7811..8140 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7991..8126 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8053..8072 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8188..8235 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8254..8628 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8758..8879 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8795..8814) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8849..8868 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8928..8944) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8928..8944) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8941..8963) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8952..8968 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8976..9006) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9021..9042 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9159..9176) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9330..9918) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9410..9429) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10089..10949) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10712..10731 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10950..11054) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag 3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt 3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccc 3901 cgggtaccgg taggccacca tgtcgcggag gccatggctt cttgccctag cgctggcagt 3961 ggcgctggcg gccggcagcg caggagcctc gactggcagt gacgcgacgg tgccggtcgc 4021 gactcaggat ggccccgact acgttttcca ccgtgcccac gagcgcatgc tcttccaaac 4081 ctcatacact cttgagaaca atggttctgt tatttgcatc ccgaacaacg gccagtgctt 4141 ctgcttggct tggcttaaat ccaacggaac aaatgccgag aagttggctg ccaacattct 4201 gcagtggatt acttttgcgc tttcagcgct ctgcctgatg ttctacggct accagacctg 4261 gaagtctact tgcggctggg aggagattta cgtggccacg atcgagatga tcaagttcat 4321 catcgagtat ttccatgagt ttgacgaacc tgcggtgatc tactcatcca acggcaacaa 4381 gaccgtgtgg cttcgttacg cggagtggct gctgacctgc cctgtcattc ttatccatct 4441 gagcaacctt acgggtctgg cgaacgacta taacaagcgt accatgggtc tgctggtgtc 4501 agatatcggc acgatcgtgt ggggcaccac ggccgcgctg tccaagggat acgtccgtgt 4561 cattttcttc ctgatgggcc tgtgctacgg catctacaca ttcttcaacg cagccaaggt 4621 ctacattgag gcgtaccaca ccgtgccgaa gggccggtgt cgccaggtgg tgactggcat 4681 ggcttggctc ttcttcgtat catggggtat gttccccatc ctgttcatcc tcggccccga 4741 gggcttcggc gtcctgagcg tgtacggctc caccgtcggc cacaccatca ttgacctgat 4801 gtcgaagaac tgctggggtc tgctcggcca ctacctgcgc gtgctgatcc acgagcatat 4861 cctcatccac ggcgacattc gcaagaccac caaattgaac attggtggca ctgagattga 4921 ggtcgagacg ctggtggagg acgaggccga ggctggcgcg gtcgcggccg ccaagagcag 4981 gatcaccagc gagggcgagt acatccccct ggaccagatc gacatcaacg tggtgagcaa 5041 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 5101 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 5161 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 5221 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 5281 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 5341 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 5401 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 5461 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 5521 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 5581 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 5641 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 5701 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtgag aattcgatat 5761 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5821 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5881 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5941 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6001 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6061 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6121 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 6181 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6241 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6301 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6361 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac 6421 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt 6481 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 6541 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 6601 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac 6661 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt 6721 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt 6781 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg 6841 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt 6901 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6961 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7021 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa 7081 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 7141 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 7201 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 7261 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 7321 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 7381 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 7441 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 7501 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 7561 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 7621 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 7681 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 7741 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 7801 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 7861 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 7921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 7981 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 8041 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 8101 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 8161 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc 8221 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg 8281 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc 8341 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc 8401 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc 8461 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc 8521 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc 8581 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga 8641 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 8701 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 8761 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 8821 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 8881 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 8941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 9721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 9781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 9841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 9901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 9961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 10741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 10801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 10861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 10921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 10981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac //