LOCUS Exported 10846 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.. ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-eArch 3.0-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10846) AUTHORS Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K TITLE Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. JOURNAL Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808. PUBMED 22179551 REFERENCE 2 (bases 1 to 10846) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10846 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3890 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" regulatory 3900..3909 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3906..4679 /codon_start=1 /product="light-driven proton pump archaerhodopsin-3 from Halorubrum sodomense (Chow et al., 2010)" /label=Arch /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD" CDS 4773..5486 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4815..4836) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5076..5095) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5423..5444 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5538..6126 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5591..5611) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6009..6020) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6129..6145) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6130..6146) /label=pBluescriptKS /note="For pBluescript vector" LTR 6651..6831 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6857..6874) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6863..7087 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7133..7561 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7220..7239) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7430..7451 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7570..7590) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7575..7904 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7755..7890 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7817..7836 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7952..7999 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8018..8392 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8522..8643 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8559..8578) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8613..8632 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8692..8708) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8692..8708) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8705..8727) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8716..8732 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8740..8770) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8785..8806 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8923..8940) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9094..9682) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9174..9193) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9853..10713) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10476..10495 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10714..10818) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac 3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga 3661 gcaggtttta agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt 3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg 3901 ccaccatgga ccccatcgct ctgcaggctg gttacgacct gctgggtgac ggcagacctg 3961 aaactctgtg gctgggcatc ggcactctgc tgatgctgat tggaaccttc tactttctgg 4021 tccgcggatg gggagtcacc gataaggatg cccgggaata ttacgctgtg actatcctgg 4081 tgcccggaat cgcatccgcc gcatatctgt ctatgttctt tggtatcggg cttactgagg 4141 tgaccgtcgg gggcgaaatg ttggatatct attatgccag gtacgccgac tggctgttta 4201 ccaccccact tctgctgctg gatctggccc ttctcgctaa ggtggatcgg gtgaccatcg 4261 gcaccctggt gggtgtggac gccctgatga tcgtcactgg cctcatcgga gccttgagcc 4321 acacggccat agccagatac agttggtggt tgttctctac aatttgcatg atagtggtgc 4381 tctattttct ggctacatcc ctgcgatctg ctgcaaagga gcggggcccc gaggtggcat 4441 ctacctttaa caccctgaca gctctggtct tggtgctgtg gaccgcttac cctatcctgt 4501 ggatcatagg cactgagggc gctggcgtgg tgggcctggg catcgaaact ctgctgttta 4561 tggtgttgga cgtgactgcc aaggtcggct ttggctttat cctgttgaga tcccgggcta 4621 ttctgggcga caccgaggca ccagaaccca gtgccggtgc cgatgtcagt gccgccgacc 4681 gaccggtagt agcagtgagc aaggcggccg ccaagagcag gatcaccagc gagggcgagt 4741 acatccccct ggaccagatc gacatcaacg tggtgagcaa gggcgaggag ctgttcaccg 4801 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 4861 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 4921 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt 4981 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 5041 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 5101 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 5161 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 5221 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 5281 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 5341 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 5401 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 5461 ctctcggcat ggacgagctg tacaagttct gctacgagaa cgaggtgtaa tgagaattcg 5521 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta 5581 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 5641 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 5701 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 5761 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 5821 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 5881 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt 5941 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 6001 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 6061 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 6121 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca 6181 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg 6241 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc 6301 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 6361 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact 6421 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac 6481 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc 6541 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca 6601 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct 6661 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6721 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 6781 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt 6841 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 6901 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 6961 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 7021 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 7081 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 7141 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 7201 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 7261 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 7321 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 7381 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 7441 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 7501 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 7561 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 7621 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 7681 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 7741 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 7801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 7861 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 7921 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat 7981 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt 8041 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg 8101 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct 8161 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt 8221 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga 8281 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct 8341 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct 8401 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 8461 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 8521 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 8581 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8641 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 8701 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 8761 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 8821 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 8881 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 9121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 9181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 9241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 9301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 9361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 9421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 9481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 9541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 10021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 10081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 10141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 10201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 10261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 10321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 10381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 10441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 10501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 10561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac //