LOCUS Exported 891 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Bacterial expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS DNMT3B (3QKJ) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 891) TITLE JOURNAL Unpublished REFERENCE 2 (bases 1 to 891) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..891 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(15..32) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS complement(512..532) /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS complement(545..562) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" RBS 572..594 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind 609..633 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(633..652) /label=T7 /note="T7 promoter, forward primer" promoter complement(634..652) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(736..755) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttcgtcatc 61 aaggctgggt gttattcggc ttcagcccct cgattccggt aggtttaaaa cccccgtgag 121 cccactccag cataggcttc agctggtcct ccaggctgtc acccggacta gacgggaaag 181 tcttacccgc acggacgcga gctttctcca gtgcgtggta catggcttta cgataagata 241 ccagcttgtt aaacgtcgcc agattgaaat gctgcgagaa cagtcccaga gccaccagtt 301 tgtctgcgga tacttctgag aatttcccgt caccaaacca ttggacccag cgcatgccac 361 tcatggcctg acgcttagag gtagccttcc aagacaccac catggcgggc caccaggaga 421 agcccttgat ctttccccac acgaggtccc ctattccaaa ctccttccca tcctgatact 481 ctgaactgtc tccatctcca ctgtctgcct cgccctggaa atacaagttt tctctgccgc 541 tgctgtgatg atgatgatga tgcatggtat atctccttct taaagttaaa caaaattatt 601 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg 661 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag 721 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact 781 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac 841 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc a //