LOCUS       Exported                8756 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Ptet-RFP-shR-rtTA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Naughton BJ, Han DD, Gu HH
  TITLE     Fluorescence-based evaluation of shRNA efficacy.
  JOURNAL   Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13.
  PUBMED    21726522
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   4..133
                     /label=AAV2 ITR
     protein_bind    200..218
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    271..289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    307..325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    342..360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    378..396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        408..446
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     443..467
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             483..1178
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           1191..1284
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           2583..2709
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(2803..2822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2804..2822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2835..4046
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3883..3902
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4059..4092
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     CDS             4099..4806
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    4807..4840
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     misc_feature    4859..5447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4912..4932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5330..5341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5474..5491)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5480..5704
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5790..5919
                     /label=AAV2 ITR
     primer_bind     complement(5993..6012)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(6038..6055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6209..6797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6289..6308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6968..7828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7591..7610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7829..7933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8001..8019
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8057..8079)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8179..8198
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8412..8431
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        8721..8739
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8721..8738
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggtacc cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
      241 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      301 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
      361 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
      421 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgca ccggtcgcca
      481 ccatgagcga gctgatcaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
      541 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      601 ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      661 ccagcttcat gtacggcagc aaagccttca tcaaccacac ccagggcatc cccgacttct
      721 ttaagcagtc cttccctgag ggcttcacat gggagagaat caccacatac gaagacgggg
      781 gcgtgctgac cgctacccag gacaccagct tccagaacgg ctgcatcatc tacaacgtca
      841 agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acacgcggct
      901 gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctgagaggc cacagccaga
      961 tggccctgaa gctcgtgggc gggggctacc tgcactgctc cttcaagacc acatacagat
     1021 ccaagaaacc cgctaagaac ctcaagatgc ccggcttcca cttcgtggac cacagactgg
     1081 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg gctgtggcca
     1141 agtactgcga cctccctagc aaactggggc acagataatc gatagtttgt ttgaatgagg
     1201 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
     1261 ttcaggttaa cccaacagaa ggctcgaggt atattgctgt tgacagtgag cgtgaagagc
     1321 cacagtgtgg aagatctgcc ctttcttcaa aggagtgggc tatgctgtga tcctcattgc
     1381 cctctatgtc ggcttttact acaatgtcat catcgcctgg tcactctact acctctttgc
     1441 aagcttcacc ttgaacctgc cctggaccaa ctgcggacac tcctggaaca gccccaactg
     1501 tacggacccc aaactcctca acgcctcggt gctgggtgac cataccaaat actccaaata
     1561 caagttcaca ccagctgcgg agttttatga acgcggagtc ctgcacttgc acgagagcag
     1621 tgggatccat gacatcggcc tgcctcaatg gcagctgctg ctctgcctga tggtcgtcat
     1681 cgttgtcttg tatttcagcc tctggaaagg agtgaagaca tcgggaaagg ttgtctggat
     1741 cacagccacc ctgccttact ttgtcttgtt tgtgctcctg gtccatggcg tcacactgcc
     1801 cggtgcctcc aatggcatca atgcctactt gcacattgac ttctaccgcc tcaaggaggc
     1861 cacggtatgg attgatgctg caacccagat atttttttct ctgggggctg gatttggagt
     1921 cttgattgca tttgccagtt acaataaatt tgacaacaac tgttacaggg atgctctact
     1981 gaccagcacc atcaactgtg ttaccagttt tatttctgga tttgccatct tctccatcct
     2041 tggttatatg gcccatgaac ataaggtcaa tattgaggat gttgccactg aaggagctgg
     2101 gcttgtgttt atcctgtatc cagaagccat ctctactctg tcaggatcca cgttctgggc
     2161 cgtcctgttc ttcttgatgc tcctggctct ggggctagat agttcaatgg gaggcatgga
     2221 agcagtcatc actggactgg ctgatgactt ccaggtcctg aagcgacacc ggaaactctt
     2281 cacgtgtgtt gtcaccatca gcaccttcct tctggcattg ttttgcataa caaagggagg
     2341 gatttatgtc ctcactctgc tggacacctt tgcagcaggc acctccattc tgtttgcggt
     2401 gcttatggaa gctattggag tttcctggtt ttatggtgtg gacaggttca gcaatgacat
     2461 ccagcagatg atggggttta agccaggcct gtactggagg ctgtgttgga agttcgtgag
     2521 ccccgccttc ctcctgtttg tggtggtggt cagcatcatc aacttcaagc cgctcttcat
     2581 gcctactgcc tcggaattca aggggctact ttaggagcaa ttatcttgtt tactaaaact
     2641 gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg gtataaatta
     2701 aatcactttt ttcaattgga agactaatgc ggccggccat tactccgtct cgtgtcttgt
     2761 tgcatatgtc tgctggtttg tttgatgttg tttgcgggcg ggccctatag tgagtcgtat
     2821 tacctaggac gcgtggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     2881 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
     2941 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
     3001 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
     3061 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
     3121 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
     3181 gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
     3241 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg
     3301 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
     3361 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
     3421 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
     3481 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
     3541 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     3601 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     3661 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     3721 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     3781 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     3841 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     3901 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     3961 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     4021 ggccgttttt ggcttttttg ttagacgcta gcggatccat aacttcgtat agtatacatt
     4081 atacgaagtt atgccaccat gtctaggctg gacaagagca aagtcataaa cggagctctg
     4141 gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
     4201 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
     4261 ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
     4321 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
     4381 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
     4441 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
     4501 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
     4561 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
     4621 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
     4681 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     4741 tttgacttag acatgctccc agccgatgcc cttgacgatt ttgaccttga catgctcccc
     4801 gggtaaataa cttcgtatag tatacattat acgaagttat ggatccaagc ttatcgataa
     4861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     5461 actcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5521 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5581 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5641 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5701 atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcatggct acgtagataa
     5761 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     5821 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     5881 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gctttttgca aaagcctagg
     5941 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     6001 tctgcataaa taaaaaaaat tagtcagcca tggcttcctc gctcactgac tcgctgcgct
     6061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6721 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6781 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6841 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7081 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7921 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     7981 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     8041 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     8101 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     8161 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cattcgacgc
     8221 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     8281 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     8341 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     8401 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     8461 atgccggcca cgatgcgtcc ggcgtagagg atctggctag cgatgaccct gctgattggt
     8521 tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc
     8581 aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag taagccagat
     8641 gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta atacataacc
     8701 ttatgtatca tacacatacg atttaggtga cactatagaa tacacggaat taattc
//
