LOCUS Exported 3699 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt1 STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3699) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3699) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3699 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1824..1923) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1940..1959) /label=T7 /note="T7 promoter, forward primer" promoter complement(1941..1959) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1964..1980) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1964..1980) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2082..2101) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2093..2902 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2160..2179) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3049..3637 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3538..3557 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat ggggctctgg gcgctgttgc ctggctgggt 721 ttctgctacg ctgctgctgg cgctggccgc tctgcccgca gccctggctg ccaacagcag 781 tggccgatgg tggggtattg tgaacgtagc ctcctccacg aacctgctta cagactccaa 841 gagtctgcaa ctggtactcg agcccagtct gcagctgttg agccgcaaac agcggcgtct 901 gatacgccaa aatccgggga tcctgcacag cgtgagtggg gggctgcaga gtgccgtgcg 961 cgagtgcaag tggcagttcc ggaatcgccg ctggaactgt cccactgctc cagggcccca 1021 cctcttcggc aagatcgtca accgaggctg tcgagaaacg gcgtttatct tcgctatcac 1081 ctccgccggg gtcacccatt cggtggcgcg ctcctgctca gaaggttcca tcgaatcctg 1141 cacgtgtgac taccggcggc gcggccccgg gggccccgac tggcactggg ggggctgcag 1201 cgacaacatt gacttcggcc gcctcttcgg ccgggagttc gtggactccg gggagaaggg 1261 gcgggacctg cgcttcctca tgaaccttca caacaacgag gcaggccgta cgaccgtatt 1321 ctccgagatg cgccaggagt gcaagtgcca cgggatgtcc ggctcatgca cggtgcgcac 1381 gtgctggatg cggctgccca cgctgcgcgc cgtgggcgat gtgctgcgcg accgcttcga 1441 cggcgcctcg cgcgtcctgt acggcaaccg cggcagcaac cgcgcttcgc gggcggagct 1501 gctgcgcctg gagccggaag acccggccca caaaccgccc tccccccacg acctcgtcta 1561 cttcgagaaa tcgcccaact tctgcacgta cagcggacgc ctgggcacag caggcacggc 1621 agggcgcgcc tgtaacagct cgtcgcccgc gctggacggc tgcgagctgc tctgctgcgg 1681 caggggccac cgcacgcgca cgcagcgcgt caccgagcgc tgcaactgca ccttccactg 1741 gtgctgccac gtcagctgcc gcaactgcac gcacacgcgc gtactgcacg agtgtctgtg 1801 aattctgaag ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa 1861 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat 1921 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 1981 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 2041 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca 2101 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg 2161 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg 2221 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 2281 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 2341 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac 2401 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 2461 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 2521 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 2581 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 2641 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 2701 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 2761 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 2821 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 2881 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat 2941 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc 3001 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 3061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 3121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 3181 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 3241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 3301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 3361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 3421 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga 3481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 3541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 3601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 3661 acggttcctg gccttttgct ggccttttgc tcacatgtt //