LOCUS Exported 3666 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt5B STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3666) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3666) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3666 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1791..1890) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1907..1926) /label=T7 /note="T7 promoter, forward primer" promoter complement(1908..1926) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1931..1947) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1931..1947) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2049..2068) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2060..2869 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2127..2146) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3016..3604 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3505..3524 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat gcccagcctg ctgctgctgt tcacggctgc 721 tctgctgtcc agctgggctc agcttctgac agacgccaac tcctggtggt cattagcttt 781 gaacccggtg cagagacccg agatgtttat catcggtgcc cagcccgtgt gcagtcagct 841 tcccgggctc tcccctggcc agaggaagct gtgccaattg taccaggagc acatggccta 901 cataggggag ggagccaaga ctggcatcaa ggaatgccag caccagttcc ggcagcggcg 961 gtggaattgc agcacagcgg acaacgcatc tgtctttggg agagtcatgc agataggcag 1021 ccgagagacc gccttcaccc acgcggtgag cgccgcgggc gtggtcaacg ccatcagccg 1081 ggcctgccgc gagggcgagc tctccacctg cggctgcagc cggacggcgc ggcccaagga 1141 cctgccccgg gactggctgt ggggcggctg tggggacaac gtggagtacg gctaccgctt 1201 cgccaaggag tttgtggatg cccgggagcg agagaagaac tttgccaaag gatcagagga 1261 gcagggccgg gtgctcatga acctgcaaaa caacgaggcc ggtcgcaggg ctgtgtataa 1321 gatggcagac gtagcctgca aatgccacgg cgtctcgggg tcctgcagcc tcaagacctg 1381 ctggctgcag ctggccgagt tccgcaaggt cggggaccgg ctgaaggaga agtacgacag 1441 cgcggccgcc atgcgcgtca cccgcaaggg ccggctggag ctggtcaaca gccgcttcac 1501 ccagcccacc ccggaggacc tggtctatgt ggaccccagc cccgactact gcctgcgcaa 1561 cgagagcacg ggctccctgg gcacgcaggg ccgcctctgc aacaagacct cggagggcat 1621 ggatggctgt gagctcatgt gctgcgggcg tggctacaac cagttcaaga gcgtgcaggt 1681 ggagcgctgc cactgcaagt tccactggtg ctgcttcgtc aggtgtaaga agtgcacgga 1741 gatcgtggac cagtacatct gtaaatgaat tctgaagggt gggcgcgccg acccagcttt 1801 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt 1861 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc 1921 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg 1981 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 2041 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa 2101 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 2161 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 2221 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 2281 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 2341 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 2401 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 2461 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 2521 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 2581 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca 2641 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 2701 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 2761 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 2821 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 2881 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg 2941 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa 3001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 3061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 3121 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 3181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 3241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 3301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 3361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc 3421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 3481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 3541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 3601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 3661 catgtt //