LOCUS Exported 3636 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt7A STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3636) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3636) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3636 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1761..1860) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1877..1896) /label=T7 /note="T7 promoter, forward primer" promoter complement(1878..1896) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1901..1917) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1901..1917) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2019..2038) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2030..2839 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2097..2116) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2986..3574 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3475..3494 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat gaaccggaaa gcgcggcgct gcctgggcca 721 cctctttctc agcctgggca tggtctacct ccggatcggt ggcttctcct cagtggtagc 781 tctgggcgca agcatcatct gtaacaagat cccaggcctg gctcccagac agcgggcgat 841 ctgccagagc cggcccgacg ccatcatcgt cataggagaa ggctcacaaa tgggcctgga 901 cgagtgtcag tttcagttcc gcaatggccg ctggaactgc tctgcactgg gagagcgcac 961 cgtcttcggg aaggagctca aagtggggag ccgggaggct gcgttcacct acgccatcat 1021 tgccgccggc gtggcccacg ccatcacagc tgcctgtacc cagggcaacc tgagcgactg 1081 tggctgcgac aaagagaagc aaggccagta ccaccgggac gagggctgga agtggggtgg 1141 ctgctctgcc gacatccgct acggcatcgg cttcgccaag gtctttgtgg atgcccggga 1201 gatcaagcag aatgcccgga ctctcatgaa cttgcacaac aacgaggcag gccgaaagat 1261 cctggaggag aacatgaagc tggaatgtaa gtgccacggc gtgtcaggct cgtgcaccac 1321 caagacgtgc tggaccacac tgccacagtt tcgggagctg ggctacgtgc tcaaggacaa 1381 gtacaacgag gccgttcacg tggagcctgt gcgtgccagc cgcaacaagc ggcccacctt 1441 cctgaagatc aagaagccac tgtcgtaccg caagcccatg gacacggacc tggtgtacat 1501 cgagaagtcg cccaactact gcgaggagga cccggtgacc ggcagtgtgg gcacccaggg 1561 ccgcgcctgc aacaagacgg ctccccaggc cagcggctgt gacctcatgt gctgtgggcg 1621 tggctacaac acccaccagt acgcccgcgt gtggcagtgc aactgtaagt tccactggtg 1681 ctgctatgtc aagtgcaaca cgtgcagcga gcgcacggag atgtacacgt gcaagtgaat 1741 tctgaagggt gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca 1801 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 1861 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca 1921 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc 1981 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat 2041 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta 2101 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa 2161 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac 2221 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca 2281 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc 2341 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt 2401 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt 2461 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt 2521 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt 2581 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt 2641 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata 2701 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg 2761 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat 2821 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac 2881 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc 2941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 3001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 3061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 3121 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 3181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 3241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 3301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 3361 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag 3421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 3481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 3541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 3601 gttcctggcc ttttgctggc cttttgctca catgtt //