LOCUS Exported 3684 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt16 STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3684) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3684) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3684 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1809..1908) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1925..1944) /label=T7 /note="T7 promoter, forward primer" promoter complement(1926..1944) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1949..1965) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1949..1965) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2067..2086) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2078..2887 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2145..2164) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3034..3622 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3523..3542 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat ggacagggcg gcgctcctgg gactggcccg 721 cttgtgcgcg ctgtgggcag ccctgctcgt gctgttcccc tacggagccc aaggaaactg 781 gatgtggttg ggcattgcct ccttcggggt tccagagaag ctgggctgcg ccaatttgcc 841 gctgaacagc cgccagaagg agctgtgcaa gaggaaaccg tacctgctgc cgagcatccg 901 agagggcgcc cggctgggca ttcaggagtg cgggagccag ttcagacacg agagatggaa 961 ctgcatgatc accgccgccg ccactaccgc cccgatgggc gccagccccc tctttggcta 1021 cgagctgagc agcggcacca aagagacagc atttatttat gctgtgatgg ctgcaggcct 1081 ggtgcattct gtgaccaggt catgcagtgc aggcaacatg acagagtgtt cctgtgacac 1141 caccttgcag aacggcggct cagcaagtga aggctggcac tgggggggct gctccgatga 1201 tgtccagtat ggcatgtggt tcagcagaaa gttcctagat ttccccatcg gaaacaccac 1261 gggcaaagaa aacaaagtac tattagcaat gaacctacat aacaatgaag ctggaaggca 1321 ggctgtcgcc aagttgatgt cagtagactg ccgctgccac ggagtttccg gctcctgtgc 1381 tgtgaaaaca tgctggaaaa ccatgtcttc ttttgaaaag attggccatt tgttgaagga 1441 taaatatgaa aacagtatcc agatatcaga caaaacaaag aggaaaatgc gcaggagaga 1501 aaaagatcag aggaaaatac caatccataa ggatgatctg ctctatgtta ataagtctcc 1561 caactactgt gtagaagata agaaactggg aatcccaggg acacaaggca gagaatgcaa 1621 ccgtacatca gagggtgcag atggctgcaa cctcctctgc tgtggccgag gttacaacac 1681 ccatgtggtc aggcacgtgg agaggtgtga gtgtaagttc atctggtgct gctatgtccg 1741 ttgcaggagg tgtgaaagca tgactgatgt ccacacttgc aagtgaattc tgaagggtgg 1801 gcgcgccgac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat 1861 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga 1921 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt 1981 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa 2041 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 2101 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 2161 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc 2221 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 2281 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 2341 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt 2401 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 2461 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 2521 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 2581 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 2641 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 2701 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 2761 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 2821 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 2881 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 2941 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc 3001 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 3061 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3181 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3421 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 3601 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 3661 ttgctggcct tttgctcaca tgtt //