LOCUS Exported 3840 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt10A Non-STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3840) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3840) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3840 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1965..2064) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2081..2100) /label=T7 /note="T7 promoter, forward primer" promoter complement(2082..2100) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2105..2121) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2105..2121) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2223..2242) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2234..3043 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2301..2320) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3190..3778 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3679..3698 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat gggcagcgcc caccctcgcc cctggctgcg 721 gctccgaccc cagccccagc cgcggccagc gctctgggtg ctcctgttct tcctactgct 781 gctggctgct gccatgccca ggtcagcacc caatgacatt ctggacctcc gcctcccccc 841 ggagcccgtg ctcaatgcca acacagtgtg cctaacattg ccaggcctga gccggcggca 901 gatggaggtg tgtgtgcgtc accctgatgt ggctgcctca gccatacagg gcatccagat 961 cgccatccac gaatgccaac accaattcag ggaccagcgc tggaactgct caagcctgga 1021 gactcgcaac aagatcccct atgagagtcc catcttcagc agaggtttcc gagagagcgc 1081 ttttgcctac gccatcgcag cagctggcgt ggtgcacgcc gtgtccaatg cgtgtgccct 1141 gggcaaactg aaggcctgtg gctgtgatgc gtcccggcga ggggacgagg aggccttccg 1201 taggaagctg caccgcttac aactggatgc actgcagcgt ggtaagggcc tgagccatgg 1261 ggtcccggaa cacccagccc tgcccacagc cagcccaggc ctgcaggact cctgggagtg 1321 gggcggctgc agccccgaca tgggcttcgg ggagcgcttt tctaaggact ttctggactc 1381 ccgggagcct cacagagaca tccacgcgag aatgaggctt cacaacaacc gagttgggag 1441 gcaggcagtg atggagaaca tgcggcggaa gtgcaagtgc cacggcacgt caggcagctg 1501 ccagctcaag acgtgctggc aggtgacgcc cgagttccgc accgtggggg cgctgctgcg 1561 cagccgcttc caccgcgcca cgctcatccg gccgcacaac cgcaacggcg gccagctgga 1621 gccgggccca gcgggggcac cctcgccggc tccgggcgct cccgggccgc gccgacgggc 1681 cagccccgcc gacctggtct acttcgaaaa gtctcccgac ttctgcgagc gcgagccgcg 1741 cctggactcg gcgggcaccg tgggccgcct gtgcaacaag agcagcgccg gctcggatgg 1801 ctgcggcagc atgtgctgcg gccgcggcca caacatcctg cgccagacgc gcagcgagcg 1861 ctgccactgc cgcttccact ggtgctgttt cgtggtctgc gaagagtgcc gcatcaccga 1921 gtgggtcagc gtctgcaagt gcattctgaa gggtgggcgc gccgacccag ctttcttgta 1981 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 2041 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta 2101 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat 2161 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 2221 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa 2281 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc 2341 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa 2401 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt 2461 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac 2521 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga 2581 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa 2641 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa 2701 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt 2761 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga 2821 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg 2881 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga 2941 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat 3001 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg 3061 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa 3121 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3301 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 3361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct 3601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt //