LOCUS Exported 3651 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Wnt11 Non-STOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3651) AUTHORS Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML TITLE A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. JOURNAL Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PUBMED 22784633 REFERENCE 2 (bases 1 to 3651) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3651 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1776..1875) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1892..1911) /label=T7 /note="T7 promoter, forward primer" promoter complement(1893..1911) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1916..1932) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1916..1932) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2034..2053) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2045..2854 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2112..2131) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3001..3589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3490..3509 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcaccat gagggcgcgg ccgcaggtct gcgaggcgct 721 gctcttcgcc ctggcgctcc agaccggcgt gtgctatggc atcaagtggc tggcgctgtc 781 caagacacca tcggccctgg cactgaacca gacgcaacac tgcaagcagc tggagggtct 841 ggtgtctgca caggtgcagc tgtgccgcag caacctggag ctcatgcaca cggtggtgca 901 cgccgcccgc gaggtcatga aggcctgtcg ccgggccttt gccgacatgc gctggaactg 961 ctcctccatt gagctcgccc ccaactattt gcttgacctg gagagaggga cccgggagtc 1021 ggccttcgtg tatgcgctgt cggccgccgc catcagccac gccatcgccc gggcctgcac 1081 ctccggcgac ctgcccggct gctcctgcgg ccccgtccca ggtgagccac ccgggcccgg 1141 gaaccgctgg ggaggatgtg cggacaacct cagctacggg ctcctcatgg gggccaagtt 1201 ttccgatgct cctatgaagg tgaaaaaaac aggatcccaa gccaataaac tgatgcgtct 1261 acacaacagt gaagtgggga gacaggctct gcgcgcctct ctggaaatga agtgtaagtg 1321 ccatggggtg tctggctcct gctccatccg cacctgctgg aaggggctgc aggagctgca 1381 ggatgtggct gctgacctca agacccgata cctgtcggcc accaaggtag tgcaccgacc 1441 catgggcacc cgcaagcacc tggtgcccaa ggacctggat atccggcctg tgaaggactc 1501 ggaactcgtc tatctgcaga gctcacctga cttctgcatg aagaatgaga aggtgggctc 1561 ccacgggaca caagacaggc agtgcaacaa gacatccaac ggaagcgaca gctgcgacct 1621 tatgtgctgc gggcgtggct acaaccccta cacagaccgc gtggtcgagc ggtgccactg 1681 taagtaccac tggtgctgct acgtcacctg ccgcaggtgt gagcgtaccg tggagcgcta 1741 tgtctgcaag tgcattctga agggtgggcg cgccgaccca gctttcttgt acaaagttgg 1801 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 1861 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat 1921 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 1981 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 2041 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc 2101 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 2161 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 2221 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct 2281 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 2341 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 2401 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 2461 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 2521 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 2581 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 2641 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 2701 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 2761 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 2821 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca 2881 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa 2941 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3001 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3061 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 3181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3361 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg 3421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t //