LOCUS Exported 3622 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUC-STS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3622) AUTHORS Watts KT, Lee PC, Schmidt-Dannert C TITLE Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. JOURNAL BMC Biotechnol. 2006 Mar 21;6:22. PUBMED 16551366 REFERENCE 2 (bases 1 to 3622) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3622 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1262..1281) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1381..1403 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1441..1459) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1527..1631 /gene="bla" /label=AmpR promoter CDS 1632..2492 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1850..1869) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2663..3251 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3152..3171 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3405..3422 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3539..3560 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3575..3605 /label=lac promoter /note="promoter for the E. coli lac operon" ORIGIN 1 ctagaagaag gaggattaca aaatggtgtc tgtgagtgag atccgcaaag ttcaaagggc 61 agaaggccct gcaactgtat tggcgatagg cacagcaaat ccaccaaatt gtattgatca 121 gagcacatat gctgattatt attttagagt aactaacagt gaacacatga ctgatctcaa 181 gaagaagttt cagcgcattt gtgagagaac acaaatcaag aacagacata tgtacttaac 241 agaagagata ctgaaagaga atcctaacat gtgcgcatac aaagcaccgt cgttggatgc 301 aagggaagac atgatgatca gggaggtacc aagggttgga aaagaggctg caaccaaggc 361 catcaaggaa tggggtcagc caatgtctaa gatcacacat ttgatcttct gcaccaccag 421 cggtgttgca ttgcctggcg ttgattacga actcatcgta ctcttaggac tcgacccatc 481 cgtcaagagg tacatgatgt accaccaagg ctgcttcgcc ggtggcactg tccttcgttt 541 ggctaaggac ttggctgaaa acaacaagga tgctcgtgtg cttatcgttt gttctgagaa 601 taccgcagtc actttccgtg gtcctagtga gacagacatg gatagtcttg tagggcaagc 661 cttgtttgct gatggagctg ctgcgattat cattggttct gatcctgtgc cagaggttga 721 aaagcctatc tttgaaattg tttcgactga tcaaaaactt gtccctaaca gccatggagc 781 catcggtggt ctccttcgtg aagttgggct tacattctat cttaataaga gtgttcctga 841 tattatttca caaaacatca atgatgcgct cagtaaagct tttgatccat tgggtatatc 901 tgattataac tcaatatttt ggattgcaca ccctggtgga cgtgcaattt tggatcaggt 961 tgaacagaaa gtgaacttga aaccagagaa gatgaatgcc actagagacg tgcttagcaa 1021 ttacggtaac atgtcaagtg cgtgtgtgtt cttcatcatg gatttaatga ggaagaagtc 1081 ccttgaagaa ggacttaaaa ctaccggtga aggacttgat tggggcgtac tttttggctt 1141 tggtcctggt ctcactattg aaactgttgt tctccgcagc atggccatat aagcggccgc 1201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 1261 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 1321 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 1381 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 1501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 1561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 1681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 1741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 1801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 1861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 1921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 1981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 2041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 2101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 2161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 2521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 2581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 3361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 3421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 3481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 3541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 3601 tgttgtgtgg aattgtgagc gt //