LOCUS       Exported                 830 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLCA.66/2272
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, 
            Yuan W, Piston DW, Magnuson MA
  TITLE     Quantification of factors influencing fluorescent protein expression
            using RMCE to generate an allelic series in the ROSA26 locus in 
            mice.
  JOURNAL   Dis Model Mech. 2011 Feb 14. ():.
  PUBMED    21324933
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(28..61)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        94..593
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(129..152)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     526..545
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 tctctcgagg ctagcatgca tatcgattac cgttcgtata atgtatgcta tacgaagtta
       61 tactagtaag cttgatatcg aattaattct accgggtagg ggaggcgctt ttcccaaggc
      121 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
      181 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
      241 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
      301 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
      361 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
      421 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
      481 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
      541 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
      601 gccaacgcca ccatggggac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
      661 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
      721 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
      781 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
//
