LOCUS       Exported                 751 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, 
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced 
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
  PUBMED    21952217
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      255..326
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      342..369
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        582..611
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    619..635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 catgcaacgg ccccaggcac caatgcccgc caactggccc gtatcgtggc ctgcgcggtt
       61 ctggcgggtg agctgagcct gtgcgccgca ttagccgcgg gccatttagt tcaatctcac
      121 atgacccaca accgcaagcc ggcagaacca accaagccaa ataacctgga cgcaaccgac
      181 attaaccgtc tgaaggatgg cagcgtcacg tgcattaaaa gctgatgagg atctttttaa
      241 ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      301 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      361 ctttctgcgt ttatagcgaa ttggatctgg tttgacagct tatcatcgac tgcacggtgc
      421 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
      481 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
      541 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatccgg
      601 ctcgtataat gtgtggaatt gtgagcggat aacaatttca ggatctagga ggaaataacc
      661 atgtctctgc cattcctgac gtctgcgcca ggtaaggtga tcatcttcgg cgagcactct
      721 gcggtgtaca ataagccggc cgtcgccgcc t
//
