LOCUS Exported 4904 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION In vivo visualization of golgi apparatus (can be used for colocalization studies). ACCESSION . VERSION . KEYWORDS pmTurquoise2-Golgi SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4904) AUTHORS Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A TITLE Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. JOURNAL Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. PUBMED 22434194 REFERENCE 2 (bases 1 to 4904) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4904 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 844..853 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 850..1569 /codon_start=1 /product="enhanced monomeric variant of CFP (Goedhart et al., 2012)" /label=mTurquoise2 /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(895..916) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1156..1175) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1503..1524 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1692..1813 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1729..1748) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1783..1802 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1820..2275) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1957..1978) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2169..2188 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2302..2406 /gene="bla" /label=AmpR promoter promoter 2408..2765 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2431..2451) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2616..2751 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2678..2697 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2800..3594 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2854..2873) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3464..3483 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3782..3801) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3826..3873 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4202..4790 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4691..4710 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct cgccaccatg aggcttcggg agccgctcct gagcggcagc 661 gccgcgatgc caggcgcgtc cctacagcgg gcctgccgcc tgctcgtggc cgtctgcgct 721 ctgcaccttg gcgtcaccct cgtttactac ctggctggcc gcgacctgag ccgcctgccc 781 caactggtcg gagtctccac accgctgcag tcgacggtac cgcgggcccg ggatccaccg 841 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 901 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 961 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 1021 tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac 1081 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 1141 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1201 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1261 ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac cgccgacaag 1321 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg 1381 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1441 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat 1501 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1561 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt 1621 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 1681 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 1801 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 1861 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 1921 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 1981 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 2041 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 2101 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 2161 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 2221 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt 2281 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 2341 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 2401 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt 2461 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2521 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2581 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 2641 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 2701 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 2761 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 2821 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 2881 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 2941 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 3001 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 3061 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 3121 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 3181 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 3241 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 3301 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 3361 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 3421 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 3481 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 3541 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 3601 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 3661 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 3721 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa 3781 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 3841 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 3901 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 3961 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 4021 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc 4081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 4141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 4201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4321 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 4381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 4441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 4501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 4561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 4801 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 4861 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat //