LOCUS       Exported                 870 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCS2+8CmCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        43..61
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     43..60
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             147..857
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(292..310)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     560..579
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt gacactatag
       61 aatacaagct acttgttctt tttgcaggat ccactagtgg cgcgccatta attaaaggcc
      121 ggccagcgat cgccggacat cgattgatgg tgagcaaggg cgaggaggat aacatggcca
      181 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      241 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      301 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      361 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      421 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      481 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      541 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      601 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      661 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      721 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      781 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      841 acgagctgta caagtaagaa ttcaaggcct
//
