LOCUS Exported 840 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pTrcHisA-rat-PTP1B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 840) AUTHORS Cortesio CL, Chan KT, Perrin BJ, Burton NO, Zhang S, Zhang ZY, Huttenlocher A TITLE Calpain 2 and PTP1B function in a novel pathway with Src to regulate invadopodia dynamics and breast cancer cell invasion. JOURNAL J Cell Biol. 2008 Mar 10;180(5):957-71. PUBMED 18332219 REFERENCE 2 (bases 1 to 840) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..840 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 84..110 /codon_start=1 /product="synthetic cistron containing a ribosome binding site (Shine-Dalgarno sequence), for enhancing the bacterial expression of a downstream cistron (Schoner, 1997)" /label=minicistron /note="This first cistron should terminate 3 bp upstream of the ATG for the second cistron." /translation="MYRLNKEE" CDS 126..143 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 147..179 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 183..206 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" ORIGIN 1 acaatttatc agacaatctg tgtgggcact cgaccggaat tatcgattaa ctttattatt 61 aaaaattaaa gaggtatata ttaatgtatc gattaaataa ggaggaataa accatggggg 121 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc 181 gggatctgta cgacgatgac gataaggatc gatggggatc catggaaatg gagaaggaat 241 tcgagcagat cgataaggct gggaactggg cggctattta ccaggatatt cgacatgaag 301 ccagtgactt cccatgcaga atagcgaaac ttcctaagaa caaaaaccgg aacaggtacc 361 gagatgtcag cccttttgac cacagtcgga ttaaattgca tcaggaagat aatgactata 421 tcaatgccag cttgataaaa atggaggaag cccagaggag ctatatcctc acccagggcc 481 ctttaccaaa cacgtgcggg cacttctggg agatggtgtg ggagcagaag agcaggggcg 541 tggtcatgct caaccgcatc atggagaaag gctcgttaaa atgtgcccag tattggccac 601 agaaagaaga aaaagagatg gtcttcgatg acaccaattt gaagctgaca ctgatctctg 661 aagatgtcaa gtcatattac acagtacggc agttggagtt ggagaacctg gctacccagg 721 aggctcgaga gatcctgcat ttccactaca ccacctggcc tgactttgga gtccctgagt 781 cacctgcctc tttcctcaat ttcctattca aagtccgaga gtcaggctca ctcagcccag //