LOCUS Exported 834 bp ds-DNA linear SYN 26-MAY-2021 DEFINITION AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.. ACCESSION . VERSION . KEYWORDS pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 834) AUTHORS Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K TITLE Neocortical excitation/inhibition balance in information processing and social dysfunction. JOURNAL Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360. PUBMED 21796121 REFERENCE 2 (bases 1 to 834) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..834 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(236..269) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(320..353) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." intron 393..596 /label=EF-1-alpha intron A /note="truncated intron upstream of the start codon of human EF-1-alpha" primer_bind complement(416..436) /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 61 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 121 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 181 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatagggc gcgccataac 241 ttcgtataat gtatgctata cgaagttatt aagaggtttc atattgctaa tagcagctac 301 aatccagcta ccattctgca taacttcgta taaagtatcc tatacgaagt tattccggag 361 tcgactctag aggatccggt acctcacgac acctgaaatg gaagaaaaaa actttgaacc 421 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag 481 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac 541 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac 601 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag 661 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc 721 gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca 781 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggc //