LOCUS       Exported                 826 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant
            C1V1 (E122T/E162T) for fast and potent optical excitation at 
            red-shifted wavelengths..
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    39..72
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    123..156
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat agcatacatt
       61 atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct gggttaaggc
      121 aaataacttc gtataggata ctttatacga agttatgcta gccaccatgt cgcggaggcc
      181 atggcttctt gccctagcgc tggcagtggc gctggcggcc ggcagcgcag gagcctcgac
      241 tggcagtgac gcgacggtgc cggtcgcgac tcaggatggc cccgactacg ttttccaccg
      301 tgcccacgag cgcatgctct tccaaacctc atacactctt gagaacaatg gttctgttat
      361 ttgcatcccg aacaacggcc agtgcttctg cttggcttgg cttaaatcca acggaacaaa
      421 tgccgagaag ttggctgcca acattctgca gtggattact tttgcgcttt cagcgctctg
      481 cctgatgttc tacggctacc agacctggaa gtctacttgc ggctgggaga ccatttacgt
      541 ggccacgatc gagatgatca agttcatcat cgagtatttc catgagtttg acgaacctgc
      601 ggtgatctac tcatccaacg gcaacaagac cgtgtggctt cgttacgcga cctggctgct
      661 cacctgcccg gtccttctga tccatctgag caacctcaca ggcctgaagg acgattatag
      721 caaaaggact atgggcctgt tggtttctga tgtgggatgc atcgtgtggg gcgcaaccag
      781 cgccatgtgt acggggtgga cgaagatcct gttcttcctc atctca
//
