LOCUS Exported 884 bp ds-DNA linear SYN 26-MAY-2021 DEFINITION AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.. ACCESSION . VERSION . KEYWORDS pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 884) AUTHORS Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K TITLE Neocortical excitation/inhibition balance in information processing and social dysfunction. JOURNAL Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360. PUBMED 21796121 REFERENCE 2 (bases 1 to 884) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..884 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(14..47) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(98..131) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." intron 171..374 /label=EF-1-alpha intron A /note="truncated intron upstream of the start codon of human EF-1-alpha" primer_bind complement(194..214) /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 agtaaggcgc gccataactt cgtataatgt atgctatacg aagttattaa gaggtttcat 61 attgctaata gcagctacaa tccagctacc attctgcata acttcgtata aagtatccta 121 tacgaagtta ttccggagtc gactctagag gatccggtac ctcacgacac ctgaaatgga 181 agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga accaagatcc aaactcaaaa 241 agggcaaatt ccaaggagaa ttacatcaag tgccaagctg gcctaacttc agtctccacc 301 cactcagtgt ggggaaactc catcgcataa aacccctccc cccaacctaa agacgacgta 361 ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg cccggtactc cgtggagtca 421 catgaagcga cggctgagga cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc 481 cgctctcccg agcgccgcgt cctccatttt gagctccctg cagcagggcc gggaagcggc 541 catctttccg ctcacgcaac tggtgccgac cgggccagcc ttgccgccca gggcggggcg 601 atacacggcg gcgcgaggcc aggcaccaga gcaggccggc cagcttgaga ctacccccgt 661 ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca 721 cgggccccgt cgccgcccgc ggccccaaaa accgaaatac cagtgtgcag atcttggccc 781 gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa 841 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtg //