LOCUS       Exported                 892 bp ds-DNA     linear   SYN 27-MAY-2021
DEFINITION  AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for 
            ultrafast optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKIIa-hChR2(E123A)-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      226..235
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
       61 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
      121 cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag
      181 ttctgggggc agctctagag cggtaccgga tccccgggta ccggtgccac catggattat
      241 ggaggcgctt tgtctgccgt cggacgcgaa cttttgttcg ttactaatcc tgtggtggtg
      301 aacgggtccg tcctggtccc tgaggatcaa tgttactgtg ccggatggat tgaatctcgc
      361 ggcacgaacg gcgctcagac cgcgtcaaat gtcctgcagt ggcttgcagc aggattcagc
      421 attttgctgc tgatgttcta tgcctaccaa acctggaaat ctacatgcgg ctgggaggag
      481 atctatgtgt gcgccattga aatggttaag gtgattctcg agttcttttt tgagtttaag
      541 aatccctcta tgctctacct tgccacagga caccgggtgc agtggctgcg ctatgcagcc
      601 tggctgctca cttgtcctgt catccttatc cacctgagca acctcaccgg cctgagcaac
      661 gactacagca ggagaaccat gggactcctt gtctcagaca tcgggactat cgtgtggggg
      721 gctaccagcg ccatggcaac cggctatgtt aaagtcatct tcttttgtct tggattgtgc
      781 tatggcgcga acacattttt tcacgccgcc aaagcatata tcgagggtta tcatactgtg
      841 ccaaagggtc ggtgccgcca ggtcgtgacc ggcatggcat ggctgttttt cg
//
