LOCUS Exported 822 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.. ACCESSION . VERSION . KEYWORDS pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 822) AUTHORS Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K TITLE Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins. JOURNAL Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808. PUBMED 22179551 REFERENCE 2 (bases 1 to 822) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..822 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 226..235 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat 61 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct 121 cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag 181 ttctgggggc agctctagag cggtaccgga tccccgggta ccggtgccac catggactat 241 ggcggcgctt tgtctgccgt cggacgcgaa cttttgttcg ttactaatcc tgtggtggtg 301 aacgggtccg tcctggtccc tgaggatcaa tgttactgtg ccggatggat tgaatctcgc 361 ggcacgaacg gcgctcagac cgcgtcaaat gtcctgcagt ggcttgcagc aggattcagc 421 attttgctgc tgatgttcta tgcctaccaa acctggaaat ctacatgcgg ctgggaggag 481 atctatgtgt gcgccattga aatggttaag gtgattctcg agttcttttt tgagtttaag 541 aatccctcta tgctctacct tgccacagga caccgggtgc agtggctgcg ctatgcaacc 601 tggctgctca cttgtcctgt catccttatc cacctgagca acctcaccgg cctgagcaac 661 gactacagca ggagaaccat gggactcctt gtctcagaca tcgggtgtat cgtgtggggg 721 gctaccagcg ccatggcaac cggctatgtt aaagtcatct tcttttgtct tggattgtgc 781 tatggcgcga acacattttt tcacgccgcc aaagcatata tc //