LOCUS Exported 7052 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMMP-MCS-IRES-mRFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7052) AUTHORS Pratt ZL, Zhang J, Sugden B TITLE The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus Can Simultaneously Induce and Inhibit Apoptosis in B Cells. JOURNAL J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8. PUBMED 22318153 REFERENCE 2 (bases 1 to 7052) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7052 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 84..387 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 388..591 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 542..562 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 813..1170 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1235..1651 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1581..1603 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1619..1635 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1926..2477 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2070..2087) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2297..2316 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 2499..3170 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /note="mammalian codon-optimized" /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG A" primer_bind complement(2623..2641) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2891..2910 /label=mCherry-F /note="mCherry, forward primer" primer_bind 3085..3108 /label=DsRed1-C /note="DsRed1, forward primer" primer_bind complement(4433..4449) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4433..4449) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4446..4468) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4457..4473 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4481..4511) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4526..4547 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4664..4681) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4835..5423) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4915..4934) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5594..6454) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6217..6236 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6455..6559) /gene="bla" /label=AmpR promoter primer_bind 6627..6645 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(6683..6705) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 6805..6824 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7018..7040 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7032..7049 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 7033..7049 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt 61 tcatagccca tattggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 121 ccgcccaacg acccccgccc cttgacgtca ataatgacgt atgttcccat agtaacgcca 181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 361 tacgtattag tcatcgctat taccatggtg atgcggttgt ggcagtacat caatgggcgt 421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt 481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg 541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg 601 aaccggcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc 661 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 721 ttgactaccc gtcagcgggg tctttcattt ggaggttcca ccgagatttg gagacccctg 781 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcaactta tctgtgtctg 841 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac 901 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac 961 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa 1021 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt 1081 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga 1141 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac 1201 tgtgtttctg tatttgtctg aaaatatggg cccgggctag actgttacca ctcccttaag 1261 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt 1321 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg 1381 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc 1441 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc 1501 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc 1561 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct 1621 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tcttatatgg 1681 ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta ctaacagccc 1741 ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct ggagacctct 1801 ggcggcagcc taccaagaac aactggaccg accggtcaat tggttaacgc tgagcttcga 1861 agccggcgcg gccgcacgcg tatgcatgta tacctcgagg atccggaatt ccgccccccc 1921 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt 1981 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt 2041 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa 2101 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac 2161 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg 2221 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt 2281 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca 2341 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt 2401 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 2461 aaaacacgat gataatatgg ccacaaccat ggttcacctc ctccgaggac gtcatcaagg 2521 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg 2581 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 2641 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca 2701 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 2761 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 2821 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 2881 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga 2941 tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg 3001 gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc 3061 ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca 3121 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctaa ttaattaaga 3181 attccggatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga 3241 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt 3301 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag 3361 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag 3421 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc 3481 agttcctgcc ccgctcaggg ccaagaacag ttggaacagg agaatatggg ccaaacagga 3541 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc 3601 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc 3661 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc 3721 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc 3781 ctccgataga ctgcgtcgcc cgggtacccg tgttctcaat aaaccctctt gcagttgcat 3841 ccgactcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca 3901 gcggggtctt tcagtttctc ccacctacac aggtctcact aacattcctg atgtgccgca 3961 gggactccgt cagcccggtt tttgtttata ataaaatgca agaacagtgt tcccttcaag 4021 ccagactaca tcctgactct cggctttata aaagaatgtt gaagggctct gtggactatc 4081 tgccacacga ctttttaaga tttttatgcc tcctggatga gggatttagt caatctatcc 4141 tcgtctattt tgctggcttc tccgtatttt aaatttctag tttgcactcc cttcctgaga 4201 gcacggcgat tgcagagtag ttaatactct gagggcaggc ttctgtgaaa aggttgcctg 4261 ggctcagtgt gagattttgc cataaaaagg ggtcctgccc ctgtgtacag acagatcgga 4321 atctagagtg catactcaga gtccccgcgg ttccggggct ctgatctcag ggcatctttg 4381 cctagagatc ctctacgccg gacgcatcgt ggccctcgaa ttcgtaatca tggtcatagc 4441 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 4501 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 4561 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 4621 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 4681 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 4741 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 4801 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 4861 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 4921 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 4981 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 5041 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 5101 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 5161 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 5221 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 5281 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 5341 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 5401 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 5461 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 5521 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 5581 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 5641 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 5701 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 5761 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 5821 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 5881 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 5941 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 6001 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 6061 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 6121 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 6181 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 6241 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 6301 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 6361 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 6421 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 6481 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 6541 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 6601 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 6661 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 6721 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 6781 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 6841 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 6901 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 6961 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 7021 agtcacgacg ttgtaaaacg acggccagtg cc //