LOCUS       Exported                 827 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSMP-NR2F1_2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, 
            Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, 
            Daley GQ
  TITLE     Chromatin-modifying enzymes as modulators of reprogramming.
  JOURNAL   Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953.
  PUBMED    22388813
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     56..78
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     94..110
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           166..287
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           315..329
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           357..483
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(568..591)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agccctttgt cccctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
       61 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
      121 ggcgccggaa ttagatctct cgactaggga taacagggta attgtttgaa tgaggcttca
      181 gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag
      241 gttaacccaa cagaaggctc gagaaggtat attgctgttg acagtgagcg acgagctaca
      301 aagcatggga aatagtgaag ccacagatgt atttcccatg ctttgtagct cggtgcctac
      361 tgcctcggaa ttcaaggggc tactttagga gcaattatct tgtttactaa aactgaatac
      421 cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa attaaatcac
      481 ttttttcaat tggaagacta atgcgtttaa acacgcggcg acgcgttcta ccgggtaggg
      541 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      601 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      661 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      721 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      781 agtctcgtgc agatggacag caccgctgag caatggaagc gggtagg
//
