LOCUS       Exported                7702 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  EXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and
            CD4d3+4-Comp-His-Stop pentamer.
ACCESSION   .
VERSION     .
KEYWORDS    BasiginCD4d3+4-Comp-His-Stop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, 
            Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner 
            JC, Wright GJ
  TITLE     Basigin is a receptor essential for erythrocyte invasion by 
            Plasmodium falciparum.
  JOURNAL   Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606.
  PUBMED    22080952
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             764..781
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    811..866
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(865..884)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3102..3120)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3188..3292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3293..4153
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3511..3530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4324..4912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4813..4832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5066..5083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5200..5221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5236..5266
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5274..5290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5279..5301
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5298..5314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5298..5314
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5585..5964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5965..6168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6119..6139
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6165..6189
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6670..6689
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6723..6732
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact
       61 caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc
      121 ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac
      181 tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt
      241 ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta
      301 tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg
      361 tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga
      421 ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg
      481 gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc
      541 caaagggttg aattccggcg gcggctccgg aggcggtacc ggtggcggcg acctagcccc
      601 acagatgctt cgagaactcc aggagactaa tgcggcgctg caagacgtga gagagctctt
      661 gcgacagcag gtcaaggaga tcaccttcct gaagaatacg gtgatggaat gtgacgcctg
      721 cggtctagac aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca
      781 ttgaggatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
      841 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
      901 tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc
      961 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     1021 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     1081 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     1141 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     1201 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     1261 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     1321 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     1381 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     1441 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     1501 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     1561 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     1621 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     1681 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     1741 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     1801 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     1861 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     1921 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     1981 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     2041 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     2101 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     2161 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     2221 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     2281 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     2341 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     2401 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     2461 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     2521 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     2581 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     2641 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     2701 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     2761 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     2821 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     2881 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     2941 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     3001 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     3061 gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt
     3121 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3241 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3301 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3361 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3421 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3481 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     3541 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3601 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3661 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3721 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3781 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     3841 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3901 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3961 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4021 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4081 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4141 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4201 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4441 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4981 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5041 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5101 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     5161 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     5221 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     5281 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     5341 ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     5401 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     5461 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     5521 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     5581 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     5641 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5701 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5761 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5821 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     5881 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     5941 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     6001 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     6061 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     6121 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     6181 cgtcagatcc tcactctctt ccgcatcgct gtctgcgagg gccagctgtt gggctcgcgg
     6241 ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc gtcggcctcc
     6301 gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat cggaaaacct
     6361 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
     6421 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
     6481 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
     6541 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
     6601 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
     6661 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt taaactgcgg
     6721 ccgccaccat ggccgctgcc ctgttcgtgc tgctgggctt cgctctgctg ggaacccatg
     6781 gcgctagcgg cgctgccgga tttgtgcagg cccccctgag ccagcagaga tgggtcggcg
     6841 gcagcgtgga actgcactgc gaggccgtgg gcagccctgt gcctgagatc cagtggtggt
     6901 tcgagggcca gggccccaac gatacctgta gccagctgtg ggacggcgcc agactggaca
     6961 gagtgcacat ccacgccacc tatcaccagc acgccgcctc taccattagc atcgacaccc
     7021 tggtggaaga ggacaccggc acctacgagt gcagagccag caacgacccc gaccggaacc
     7081 acctgaccag agcccccaga gtgaaatggg tccgggctca ggctgtggtg ctggtgctgg
     7141 aacccggcac cgtgttcacc accgtggagg acctgggcag caagatcctg ctgacctgta
     7201 gcctgaacga cagcgccacc gaagtgaccg gccacagatg gctgaagggc ggcgtggtgc
     7261 tgaaagagga cgccctgccc ggccagaaaa ccgagttcaa ggtggacagc gacgaccagt
     7321 ggggcgagta cagctgcgtg ttcctgcccg agcctatggg caccgccaat atccagctgc
     7381 acggccctcc aagagtgaag gccgtgaagt ccagcgagca catcaacgag ggcgagacag
     7441 ccatgctcgt gtgcaagagc gagagcgtgc cccctgtgac cgactgggcc tggtacaaga
     7501 tcaccgacag cgaggacaag gccctgatga acggcagcga gagccggttc ttcgtgagca
     7561 gcagccaggg cagaagcgag ctgcacatcg agaacctgaa catggaagcc gaccccggcc
     7621 agtacagatg caacggcacc agcagcaagg gctccgacca ggccatcatc accctgagag
     7681 tgcggtcaca cctgggcgcg cc
//
