LOCUS       Exported                 766 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223-PRKD2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Nov 24. ():.
  PUBMED    21107320
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    17..116
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aatatcagct gggatggcaa taatgatttt attttgactg atagtgacct gttcgttgca
       61 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ttggcaagag
      121 aacactgatg cgctccgcca gcccctgcat gtcgtggtcc tgtggtggac aggccccacc
      181 gagatccctg tccgtgggca gcccagaccc aggcagcgga tgctctgctg caaactgctc
      241 ccagcgcgcg tcgtcactct catgcgtgat gtatcgctct cccatcttcc cctccagctc
      301 tcggaggtcc agccacgtct ggtactcctg taaccagggg tggctgagag atttgtccac
      361 gctgtagcgt ttgcgcatct tcacctgcag caggttgttg atgaggtcaa tggctccagc
      421 tgagatgtgg ctccaggggc tggccgggta catgaaggcg gcgttctgga tctggtcatt
      481 gatgtcctca tcctcgttga aagggaaggt gccgctgagg ctgacgtaca tgatcacgcc
      541 cactgaccac atgtccagcg agcggttgta gccctggttg agcagcacct cgggtgccag
      601 gtaggccggc gtgcccacca ctgagcggcg gaacgacttc tcgccgatga tgcgagcaaa
      661 gccaaagtca cacagcttca cctgaggaaa tgggtctgct gatgccagca acacgttttc
      721 tggtttcaag tcacagtgga caatgttctt gaagtgaagg tgtctc
//
