LOCUS Exported 7728 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pQC TdTomato IX SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7728) AUTHORS Beier KT, Samson ME, Matsuda T, Cepko CL TITLE Conditional expression of the TVA receptor allows clonal analysis of descendents from Cre-expressing progenitor cells. JOURNAL Dev Biol. 2011 May 15;353(2):309-20. Epub 2011 Mar 21. PUBMED 21397594 REFERENCE 2 (bases 1 to 7728) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7728 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 310..513 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 514..689 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 752..951 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1152..1568 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1498..1520 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1536..1552 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" enhancer 1605..1908 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1909..2112 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2109..2133 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2283..2292 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2289..3719 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" misc_feature 3728..4300 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3895..3912) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4122..4141 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" LTR 4434..4859 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" primer_bind complement(4962..4984) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5120..5140) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5125..5454 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5305..5440 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5367..5386 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 5476..5495 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(5576..5593) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5747..6335) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5827..5846) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6506..7366) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7129..7148 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7367..7471) /gene="bla" /label=AmpR promoter primer_bind 7539..7557 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 7657..309 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 1561 taggcgccgg aattgaagat ctgggggatc gatcctctag agtccgttac ataacttacg 1621 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 1681 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 1741 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 1801 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 1861 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 1921 tggcagtaca tcaatgggcg tgaatagcgg tttgactcac ggggatttcc aagtctccac 1981 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 2041 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 2101 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2161 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga 2221 acgctgcagg aattgatccg cggccgcacc ggtaggcctc gtacgcttaa ttaacggatc 2281 ccgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc 2341 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2401 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg 2461 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg 2521 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2581 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca 2641 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc 2701 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc 2761 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt 2821 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca 2881 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct 2941 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct 3001 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca 3061 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg 3121 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc 3181 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc 3241 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc 3301 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg 3361 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg 3421 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg 3481 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg 3541 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg 3601 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 3661 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag 3721 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 3781 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 3841 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 3901 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 3961 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 4021 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 4081 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 4141 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 4201 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa 4261 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgatcac gcgtctcgag 4321 atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa gcctatagag 4381 tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa 4441 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa 4501 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagggtcg 4561 accctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 4621 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 4681 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 4741 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 4801 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 4861 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 4921 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 4981 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 5041 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 5101 ggagtgtaga tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 5161 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 5281 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 5341 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 5401 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt 5461 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 5521 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 5581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6181 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 6241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 6301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac 6841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 7141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7501 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 7561 ttcgtcttca agaattagct tggccattgc atacgttgta tccatatcat aatatgtaca 7621 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt 7681 aatagtaatc aattacgggg tcattagttc atagcccata tatggagt //