LOCUS Exported 7004 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pQC NLS mKorange IX SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7004) AUTHORS Beier KT, Samson ME, Matsuda T, Cepko CL TITLE Conditional expression of the TVA receptor allows clonal analysis of descendents from Cre-expressing progenitor cells. JOURNAL Dev Biol. 2011 May 15;353(2):309-20. Epub 2011 Mar 21. PUBMED 21397594 REFERENCE 2 (bases 1 to 7004) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7004 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 310..513 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 514..689 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 752..951 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1152..1568 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1498..1520 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1536..1552 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" enhancer 1605..1908 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1909..2112 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2109..2133 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2297..2317 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2318..2974 /codon_start=1 /product="Kusabira-Orange fluorescent protein (Karasawa et al., 2004)" /label=Kusabira-Orange /translation="MVSVIKPEMKMKYFMDGSVNGHEFTVEGEGTGKPYEGHQEMTLRV TMAKGGPMPFSFDLVSHTFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLQFEDGGFA AVSAHISLRGNCFEHKSKFVGVNFPADGPVMQNQSSDWEPSTEKITTCDGVLKGDVTMY LKLAGGGNHKCQFKTTYKAAKKILKMPQSHFIGHRLVRKTEGNITELVEDAVAHC" misc_feature 3004..3576 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3171..3188) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3398..3417 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" LTR 3710..4135 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" primer_bind complement(4238..4260) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4396..4416) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4401..4730 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4581..4716 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4643..4662 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 4752..4771 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(4852..4869) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5023..5611) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5103..5122) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5782..6642) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6405..6424 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6643..6747) /gene="bla" /label=AmpR promoter primer_bind 6815..6833 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 6933..309 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc 241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact 541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct 601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga 661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg 721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt 781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact 841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc 901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt 961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc 1561 taggcgccgg aattgaagat ctgggggatc gatcctctag agtccgttac ataacttacg 1621 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 1681 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 1741 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 1801 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 1861 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 1921 tggcagtaca tcaatgggcg tgaatagcgg tttgactcac ggggatttcc aagtctccac 1981 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 2041 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 2101 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2161 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga 2221 acgctgcagg aattgatccg cggccgcacc ggtaggcctc gtacgcttaa ttaacggatc 2281 cgccaccatg actgctccaa agaagaagcg taaggtaatg gtgagtgtga ttaaaccaga 2341 gatgaagatg aagtacttca tggacggctc cgtcaatggg catgagttca cagttgaagg 2401 tgaaggcaca ggcaaacctt acgagggaca tcaagagatg acactacgcg tcacaatggc 2461 caagggcggg ccaatgcctt tctcgtttga cttagtgtca cacacgttct gttacggcca 2521 cagacctttt actaaatatc cagaagagat accagactat ttcaaacaag catttcctga 2581 aggcctgtca tgggaaaggt cgttgcagtt cgaagatggt gggttcgctg cagtcagtgc 2641 gcatataagc cttagaggaa actgcttcga gcacaaatcc aaatttgttg gggttaactt 2701 tcctgccgat ggtcctgtga tgcaaaacca aagttctgat tgggagccat caaccgagaa 2761 aattactacc tgcgacggag ttctgaaggg tgatgttacg atgtacctaa aacttgcggg 2821 aggcggcaat cacaaatgcc aattcaagac tacttacaag gcggcaaaaa agattcttaa 2881 aatgccacaa agccatttca tcgggcatcg cctcgtcagg aaaaccgaag gcaacattac 2941 tgagctggta gaagatgcag tagctcattg ctgaagcggc cgcactcctc aggtggaatt 3001 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg 3061 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 3121 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 3181 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 3241 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 3301 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 3361 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 3421 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 3481 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac 3541 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gatcacgcgt ctcgagatat 3601 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg 3661 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc 3721 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata 3781 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc 3841 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat 3901 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc 3961 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc 4021 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt 4081 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg 4141 gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag 4201 ctggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 4261 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 4321 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag 4381 tgtagatccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 4441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 4501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 4561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 4621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 4681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttactg 4741 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 4801 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 4861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 4921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 4981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 5161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 5221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 5281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 5341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 5401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 5461 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 5521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 5581 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 5641 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 5701 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 5761 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 5821 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 5881 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 5941 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 6001 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 6061 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 6121 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 6181 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 6241 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 6301 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 6361 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 6421 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 6481 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 6541 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 6601 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 6661 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 6721 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 6781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 6841 tcttcaagaa ttagcttggc cattgcatac gttgtatcca tatcataata tgtacattta 6901 tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata 6961 gtaatcaatt acggggtcat tagttcatag cccatatatg gagt //