LOCUS Exported 788 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pHL600 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 788) AUTHORS Adamson DN, Lim HN TITLE Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. JOURNAL Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PUBMED 23878244 REFERENCE 2 (bases 1 to 788) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..788 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 79..97 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" protein_bind 118..136 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 143..161 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" terminator 560..631 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 701..717 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 61 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtcc 121 ctatcagtga tagagattga catccctatc agtgatagag atactgtcga cgagtcagac 181 aacgaagtga acatcaggat gatgacactt ctgcaggaca caccaggatg gtgtttcagg 241 gaaaggcttc tggatgaagc gaagaggatg acgcaggacg cgttaaagga cacctccagg 301 atggagaatg agaaccggtc aggatgattc ggtgggtcag gaaggccagg gacacttcag 361 gatgaagtat cacatcgggg tggtgtgagc aggaagcaat agttcaggat gaacgattgg 421 ccgcaaggcc agaggaaaag ttgtcaagga tgagcaggga gcaacaaaag tagctggaat 481 gctgcgaaac gaaccgggag cgctgtgaat acagtgctcc ctttttttat tgggcccatg 541 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 601 ttttatctgt tgtttgtcgg tgaacgctct cnnnnnnngg acaaatccgc cncnnnnnac 661 ctangcgcgg atccataaat gtgancggat ancattgaca ttgtgagcgg ataacaagat 721 actcccggnn anngangaaa anaaaatgct gattctnacn cnnnnagttg gngagaccct 781 cangantg //