LOCUS Exported 746 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 746) AUTHORS Saunders A, Johnson CA, Sabatini BL TITLE Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons. JOURNAL Front Neural Circuits. 2012 Jul 27;6:47. doi: 10.3389/fncir.2012.00047. eCollection 2012. PUBMED 22866029 REFERENCE 2 (bases 1 to 746) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..746 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 47..80 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 131..164 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS complement(217..468) /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VC155 /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" primer_bind complement(259..280) /label=EGFP-C /note="EGFP, forward primer" primer_bind 608..627 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag 61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg 121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc gctaccggtc 181 gccaccgcca ccttacacct cgttctcgta gcagaacttg tacagctcgt ccatgccgag 241 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg 301 gtctttgctc agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc 361 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat 421 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata 481 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt 541 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc 601 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc 661 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa 721 gcactgcagg ccgtagccga aggtgg //