LOCUS Exported 863 bp ds-DNA linear SYN 02-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS UQ4802 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 863) AUTHORS Kerby RL, Youn H, Roberts GP TITLE RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. JOURNAL J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. PUBMED 18326575 REFERENCE 2 (bases 1 to 863) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..863 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 51..79 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 87..103 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 92..114 /label=M13/pUC Reverse /note="In lacZ gene" ORIGIN 1 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta 61 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag 121 aattcaggag gaatttaaaa tgaaatcgtc tgagcccgct tccgtatcgg cggccgagcg 181 ccgcgcggag acgttccagc acaagctcga gcagttcaat ccggggatcg tctggctgga 241 ccagcacggc cgcgtcacgg cattcaacga cgtcgcgctg caaattctcg ggccggccgg 301 cgagcaatcg ctaggcgtgg cgcaggatag tctgttcggc atcgacgtgg tgcaactgca 361 cccggagaaa agccgcgaca agctgcgctt cctgctgcag tcgaaagacg tgggtggctg 421 cccggtcaag tcgccgccgc cggtggcgat gatgatcaat attcccgacc ggatcctgat 481 gatcaaggtg tccagcatga tcgccgccgg cggcgcgtgc ggcacctgca tgatcttcta 541 cgacgtcacc gatctgacca ccgagccctc cggcctgccg gcgggcggca gcgcgccctc 601 gccgaggcgc ctcttcaaga ttcccgtgta ccggaagaac cgcgtgatcc tgctcgatct 661 gaaagacatc gtgcgctttc agggcgacgg ccactacacg acgatcgtga cgcgggacga 721 ccgctacctg tccaatcttt cactggccga tctggagctg cgtctcgaca gcagcattta 781 tctgcgcgtt catcgcagcc atatcgtgag cctgcaatat gcggtggagc tggtcaaact 841 ggacgagagc gtcaatctcg tca //