LOCUS Exported 893 bp ds-DNA linear SYN 02-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS UQ4884 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 893) AUTHORS Kerby RL, Youn H, Roberts GP TITLE RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. JOURNAL J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. PUBMED 18326575 REFERENCE 2 (bases 1 to 893) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..893 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 51..79 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 87..103 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 92..114 /label=M13/pUC Reverse /note="In lacZ gene" ORIGIN 1 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta 61 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag 121 aattcaggag gaatttaaaa tgaaatcgtc agaatccgcc gccgcgacgg cttccgagcg 181 ccgtgcagag acgttccagc acaagctcga acagttcaac ccggggatcg tctggctgga 241 cccgcagggc cacgtaagcg cattcaacga cgtcgcgctg cacattctcg ggccggccgg 301 cgagcaatcg ctcggcgtgg cgcaggatca tctgttcggg atcgacgtgg tgcaactgca 361 tccggagaaa agccgcgaca agctgcgttt tctgctgcag tccagggacg cgggtggctg 421 ccctgtcaga tcgccgccgc cggtggcgat gatgatcaac atcccggacc gcatcctgat 481 gatcaaggtg tcgaaaatga ctggcgcggc gggcacctgc ggcagctgca tgatctttta 541 cgacgtgacc gatctgacca ccgaaccgtc cagccagccg gcaggcgcca gcgtgccggc 601 gccgcgccgt ctcttcaaga tccccgtgta ccggaagagc cgcgtgatcc tgatcgatct 661 gaaggacatt gtgcgctttc agggcgacgg ccactacacg acgatcgtca cgaaagacga 721 gcgctacctc tccaatcttt cgctggccga cctcgaactg cgcctcgata gcagcgtcta 781 tctgcgggtt catcgcagcc atatcgtgag ccttccgtac gcggtcgaac tggtcaaact 841 ggacgagagc gtcaatctcg tcatggacga cgcggaacaa cccaggtacc ggt //