LOCUS Exported 921 bp ds-DNA linear SYN 04-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS TAL2228 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 921) AUTHORS Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK TITLE FLASH assembly of TALENs for high-throughput genome editing. JOURNAL Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170. PUBMED 22484455 REFERENCE 2 (bases 1 to 921) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 4, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..921 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..55 /label=T7 /note="T7 promoter, forward primer" promoter 36..54 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 80..145 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 152..172 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc 61 caagctggct agcaccatgg actacaaaga ccatgacggt gattataaag atcatgacat 121 cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcattca 181 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa 241 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg 301 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc 361 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg 421 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga 481 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg 541 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt 601 gaacctgacc ccagaccagg tagtcgcaat cgcgaacaat aatgggggaa agcaagccct 661 ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac cacggcctta caccggagca 721 agtcgtggcc attgcaagca atgggggtgg caaacaggct cttgagacgg ttcagagact 781 tctcccagtt ctctgtcaag cccacgggct gactcccgat caagttgtag cgattgcgtc 841 caacggtgga gggaaacaag cattggagac tgtccaacgg ctccttcccg tgttgtgtca 901 agcccacggt ttgacgcctg c //