LOCUS Exported 5881 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBad-Int Set Flipper SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5881) AUTHORS Bonnet J, Subsoontorn P, Endy D TITLE Rewritable digital data storage in live cells via engineered control of recombination directionality. JOURNAL Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. PUBMED 22615351 REFERENCE 2 (bases 1 to 5881) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5881 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..22 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS complement(23..901) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 928..1211 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 1134..1153 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" CDS 1265..1282 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 2795..2827 /codon_start=1 /product="C-terminal peptide that mediates degradation in bacteria through the ClpXP and ClpAP proteases (McGinness et al., 2006)" /label=ssrA tag /note="wild-type LAA variant" /translation="AANDENYALAA" misc_feature 2840..2860 /label=BioBrick suffix /note="universal suffix for all parts" rep_origin 3339..3884 /direction=RIGHT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind 3785..3804 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4001..4018 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(4468..4487) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 4479..5294 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(4552..4571) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" ORIGIN 1 gaattcgcgg ccgcttctag agttatgaca acttgacggc tacatcattc actttttctt 61 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga 121 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg 181 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa 241 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg 301 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct 361 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca 421 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc 481 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc 541 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc 601 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt 661 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc 721 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc 781 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg 841 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca 901 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt 961 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta 1021 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct 1081 ataatcacgg cagaaaagtc acattgatta tttgcacggc gtcacacttt gctatgccat 1141 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac 1201 tgtttctcca taccgttttt ttgggctagc tactagagtc acacaggaaa cctactagat 1261 gaaacatcat caccatcacc accaggccgg cagagccctg gtagtcatcc gcctgtcccg 1321 cgtcaccgat gctacgactt caccggagcg tcagctggag tcttgccagc agctctgcgc 1381 ccagcgcggc tgggacgtcg tcggggtagc ggaggatctg gacgtctccg gggcggtcga 1441 tccgttcgac cggaagcgca gaccgaacct ggcccggtgg ctagcgttcg aggagcaacc 1501 gtttgacgtg atcgtggcgt accgggtaga tcggttgacc cgatcgatcc ggcatcttca 1561 gcagctggtc cactgggccg aggaccacaa gaagctggtc gtctccgcga ccgaagcgca 1621 cttcgatacg acgacgccgt ttgcggcggt cgtcatcgcg cttatgggaa cggtggcgca 1681 gatggaatta gaagcgatca aagagcggaa ccgttcggct gcgcatttca atatccgcgc 1741 cgggaaatac cgaggatccc tgccgccgtg gggatacctg cctacgcgcg tggacgggga 1801 gtggcggctg gtgccggacc ctgtgcagcg agagcgcatc ctcgaggtgt atcaccgcgt 1861 cgtcgacaac cacgagccgc tgcatctggt ggcccacgac ctgaaccggc gtggtgtcct 1921 gtcgccgaag gactacttcg cgcagctgca aggccgcgag ccgcagggcc gggagtggtc 1981 ggctaccgcg ctgaagcgat cgatgatctc cgaggcgatg ctcgggtacg cgactctgaa 2041 cggtaagacc gtccgagacg acgacggagc cccgctggtg cgggctgagc cgatcctgac 2101 ccgtgagcag ctggaggcgc tgcgcgccga gctcgtgaag acctcccggg cgaagcccgc 2161 ggtgtctacc ccgtcgctgc tgctgcgggt gttgttctgc gcggtgtgcg gggagcccgc 2221 gtacaagttc gccgggggag gacgtaagca cccgcgctac cgctgccgct cgatggggtt 2281 cccgaagcac tgcgggaacg gcacggtggc gatggccgag tgggacgcgt tctgcgagga 2341 gcaggtactg gatctgctcg gggacgcgga gcgtctggag aaagtctggg tagcgggctc 2401 ggactccgcg gtcgaactcg cggaggtgaa cgcggagctg gtggacctga cgtcgctgat 2461 cggctccccg gcctaccggg cgggctctcc gcagcgagaa gcactggatg cccgtattgc 2521 ggcgctggcc gcgcggcaag aggagctgga gggcctggag gctcgcccgt ctggctggga 2581 gtggcgcgag accgggcagc ggttcgggga ctggtggcgg gagcaggaca ccgcggcaaa 2641 gaacacctgg cttcggtcga tgaacgttcg gctgacgttc gacgtccgcg gcgggctgac 2701 tcgcacgatc gacttcgggg atcttcagga gtacgagcag catctcaggc tcggcagcgt 2761 ggtcgaacgg ctacacaccg ggatgtcgac cggcgctgct aacgacgaaa actacgctct 2821 ggctgcttaa accggttaat actagtagcg gccgctgcag caatggcaac aacgttgcgc 2881 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 2941 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 3001 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 3061 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 3121 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 3181 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 3241 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3301 ttccactgag cgtcagaccc cttaataaga tgatcttctt gagatcgttt tggtctgcgc 3361 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc 3421 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt 3481 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt 3541 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata 3601 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt 3661 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata 3721 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc 3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc 3841 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg 3901 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc 3961 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga 4021 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct 4081 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac 4141 tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa 4201 tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg 4261 tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa 4321 gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg 4381 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa 4441 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa 4501 cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat 4561 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg 4621 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg 4681 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta 4741 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc 4801 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc 4861 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc 4921 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg 4981 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat 5041 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg 5101 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg 5161 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt 5221 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg 5281 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga 5341 cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat 5401 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa 5461 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga 5521 tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg 5581 ctcaaagatg caggggtaaa agctaaccgc atctttaccg acaaggcatc cggcagttca 5641 acagatcggg aagggctgga tttgctgagg atgaaggtgg aggaaggtga tgtcattctg 5701 gtgaagaagc tcgaccgtct tggccgcgac accgccgaca tgatccaact gataaaagag 5761 tttgatgctc agggtgtagc ggttcggttt attgacgacg ggatcagtac cgacggtgat 5821 atggggcaaa tggtggtcac catcctgtcg gctgtggcac aggctgaacg ccggaggatc 5881 c //