LOCUS Exported 4042 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSTC0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4042) AUTHORS Rodrigo G, Landrain TE, Jaramillo A TITLE De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. JOURNAL Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4. PUBMED 22949707 REFERENCE 2 (bases 1 to 4042) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4042 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 26..736 /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV TAAGITHGMDELYK" primer_bind complement(50..78) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 673..693 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" terminator 756..827 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 843..870 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 877..897 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(981..998) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1152..1740) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1232..1251) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1973..2788) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 2696..2715 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 2780..2799 /label=pENTR-R /note="pENTR vectors, reverse primer" CDS complement(2943..3803) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3566..3585 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3804..3908) /gene="bla" /label=AmpR promoter primer_bind 3976..3994 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 3998..4041 /label=bacterial terminator /note="putative bacterial transcription terminator" ORIGIN 1 ggaattcgcg gccgctgcta gccgtaaagg agaagaactt ttcactggag ttgtcccaat 61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga 121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc 181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata 241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca 301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt 361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg 421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc 481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg 541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct 601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa 661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga 721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agtagcggcc gctgcagtcc 901 ggcaaaaaaa cgggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta 961 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 1021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 1081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 1141 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 1201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 1261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 1321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 1381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 1441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 1501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 1561 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 1621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 1681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 1741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 1801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 1861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgagtccc 1921 gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa 1981 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt 2041 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg 2101 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt 2161 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg 2221 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac 2281 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag 2341 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc 2401 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta 2461 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag 2521 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga 2581 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg 2641 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc 2701 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctggagc 2761 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag 2821 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt 2881 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagctcg 2941 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 3001 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 3061 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 3121 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 3181 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 3241 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3301 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3361 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3421 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3481 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3541 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 3601 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3661 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 3721 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 3781 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3841 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3901 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 3961 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 4021 gctttcgcta aggatgattt ct //