LOCUS       Exported                 880 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    p6898 PHAGE-P CMVT N-HA-hE6AP I C820A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    29..47
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     57..81
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      168..177
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             180..206
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    222..246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcgagctct cctatcgtga tagagatctc cctatcagtg atagagatcg tcgacgagct
       61 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      121 agacaccggg accgatccag cctccggact ctagcgttta aacgctagcc accatggcgt
      181 acccatacga tgttccagat tacgctgctc gaggttatca aacaagtttg tacaaaaaag
      241 caggctccgc ggccgccccc ttcaccatga agcgagcagc tgcaaagcat ctaatagaac
      301 gctactacca ccagttaact gagggctgtg gaaatgaagc ctgcacgaat gagttttgtg
      361 cttcctgtcc aacttttctt cgtatggata ataatgcagc agctattaaa gccctcgagc
      421 tttataagat taatgcaaaa ctctgtgatc ctcatccctc caagaaagga gcaagctcag
      481 cttaccttga gaactcgaaa ggtgccccca acaactcctg ctctgagata aaaatgaaca
      541 agaaaggcgc tagaattgat tttaaagatg tgacttactt aacagaagag aaggtatatg
      601 aaattcttga attatgtaga gaaagagagg attattcccc tttaatccgt gttattggaa
      661 gagttttttc tagtgctgag gcattggtac agagcttccg gaaagttaaa caacacacca
      721 aggaagaact gaaatctctt caagcaaaag atgaagacaa agatgaagat gaaaaggaaa
      781 aagctgcatg ttctgctgct gctatggaaa gaagactcag aagcatcttc ctcaaggata
      841 ggtgatagct cacagggaga caacaatttg caaaaattag
//
