LOCUS       Exported                 802 bp ds-DNA     linear   SYN 31-MAY-2021
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    PDLIM1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 802)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             433..450
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(503..521)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      517..564
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(688..707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg accacccagc agatagacct
      121 ccagggcccg gggccgtggg gcttccgcct cgtgggcggc aaggacttcg agcagcctct
      181 cgccatttcc cgggtcactc ctggaagcaa ggcggctcta gctaatttat gtattggaga
      241 tgtaatcaca gccattgatg gggaaaatac tagcaatatg acacacttgg aagctcagaa
      301 cagaatcaaa ggctgcacag acaacttgac tctcactgta gccagatctg aacatgaatc
      361 tgacttgtga cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg
      421 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      481 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      541 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      781 gtgctttacg gcacctcgac cc
//
