LOCUS       Exported                 912 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pQC membrane CFP IX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Beier KT, Samson ME, Matsuda T, Cepko CL
  TITLE     Conditional expression of the TVA receptor allows clonal analysis of
            descendents from Cre-expressing progenitor cells.
  JOURNAL   Dev Biol. 2011 May 15;353(2):309-20. Epub 2011 Mar 21.
  PUBMED    21397594
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             196..912
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(241..262)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(502..521)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     849..870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca
       61 ttggaacgga cctgcaggaa ttgatccgcg gccgcaccgg taggcctcgt acgcttaatt
      121 aacggatccg ccgccatgct gtgctgtatg agaagaacca aacaggttga aaagaatgat
      181 gaggaccaaa agatcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      241 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      301 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      361 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
      421 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      481 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      541 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      601 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
      661 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
      721 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      781 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
      841 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      901 gagctgtaca ag
//
