LOCUS Exported 2189 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS EKAR2G_design1_mTFP_175_Venus_157 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2189) AUTHORS Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O TITLE A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. JOURNAL Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. PUBMED 23882122 REFERENCE 2 (bases 1 to 2189) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2189 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 9..26 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 36..65 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind 1571..1592 /label=EGFP-C /note="EGFP, forward primer" CDS 1650..2114 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA" primer_bind complement(1695..1716) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1956..1975) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg 61 atcttggctc aggttcagga tccggcggcc accaccgcgt tgacttcaag accatctaca 121 gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc 181 tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact 241 ccaccgacgg catggacgag ctgtacaagg gtgctagcgg aggaatggtg agcaagggcg 301 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca 361 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca 421 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc 481 tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca 541 actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg 601 acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg 661 agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag aagaagacca 721 ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg 781 tcaagcacaa gctgctgctg gagggctccg gaatggcgga cgaggagaag ctgccgcccg 841 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta 901 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg 961 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgcccg 1021 ggtctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg 1081 gcggtggtgg aggaggagga gctagaggcg gaggtggtgg aggaggtgga ggtggtggag 1141 gtggaggtgg aggtggtggc ggtggtggag gaggaggagc cagcggcgga ggtggtggag 1201 gaggtggagg tggtggaggt ggaggtggag gtggtggcgg tggtggagga ggaggaaccg 1261 gtgctgacca actgactgaa gagtggtcga cggtacagcg gaccgcgcct gacgtaccac 1321 gtacaccagt aggaaaggca aagctttcat tccaattccc gcgggcgcgg gacatcgcgg 1381 ccgcgatgca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg 1441 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 1501 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 1561 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 1621 acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag ctgttcaccg 1681 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 1741 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca 1801 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgatgt 1861 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 1921 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 1981 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 2041 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 2101 tctatatcac cgccgacaag ggtaccgata tccttcaaaa aaaacttgaa gaacttgaac 2161 ttgacgaatg agatatctaa tgactcgag //