LOCUS Exported 2171 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS EKAR2G_design1_mTFP_227_Venus_wt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2171) AUTHORS Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O TITLE A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. JOURNAL Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. PUBMED 23882122 REFERENCE 2 (bases 1 to 2171) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2171 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 9..26 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 36..65 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1386..2102 /codon_start=1 /product="yellow fluorescent protein (YFP) with fast and efficient maturation (Nagai et al., 2002)" /label=Venus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1431..1452) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1692..1711) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2039..2060 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 ccatggcaca ccatcaccac caccacggga gcggggagca gaaactaata tcagaggagg 61 atcttggctc aggttcagga tcctccaccg acggcatgga cgagctgtac aagggtgcta 121 gcggaggaat ggtgagcaag ggcgaggaga ccacaatggg cgtaatcaag cccgacatga 181 agatcaagct gaagatggag ggcaacgtga atggccacgc cttcgtgatc gagggcgagg 241 gcgagggcaa gccctacgac ggcaccaaca ccatcaacct ggaggtgaag gagggagccc 301 ccctgccctt ctcctacgac attctgacca ccgcgttcgc ctacggcaac agggccttca 361 ccaagtaccc cgacgacatc cccaactact tcaagcagtc cttccccgag ggctactctt 421 gggagcgcac catgaccttc gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca 481 tggaggagga ctccttcatc tacgagatac acctcaaggg cgagaacttc ccccccaacg 541 gccccgtgat gcagaagaag accaccggct gggacgcctc caccgagagg atgtacgtgc 601 gcgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc 661 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc 721 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg 781 tttacgagag cgccgtggcc cgcaactccg gaatggcgga cgaggagaag ctgccgcccg 841 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta 901 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg 961 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgcccg 1021 ggtctagagg cggaggtggt ggaggaggtg gaggtggtgg aggtggaggt ggaggtggtg 1081 gcggtggtgg aggaggagga gctagaggcg gaggtggtgg aggaggtgga ggtggtggag 1141 gtggaggtgg aggtggtggc ggtggtggag gaggaggagc cagcggcgga ggtggtggag 1201 gaggtggagg tggtggaggt ggaggtggag gtggtggcgg tggtggagga ggaggaaccg 1261 gtgctgacca actgactgaa gagtggtcga cggtacagcg gaccgcgcct gacgtaccac 1321 gtacaccagt aggaaaggca aagctttcat tccaattccc gcgggcgcgg gacatcgcgg 1381 ccgcgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 1441 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 1501 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc 1561 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca 1621 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 1681 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 1741 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 1801 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga 1861 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc 1921 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 1981 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 2041 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 2101 agggtaccga tatccttcaa aaaaaacttg aagaacttga acttgacgaa tgagatatct 2161 aatgactcga g //