LOCUS       Exported                 751 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBad His6 MBP TEV LIC cloning vector (8C)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 751)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca
       61 ccatggttct tctatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa
      121 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
      181 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
      241 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
      301 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
      361 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
      421 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
      481 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
      541 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
      601 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
      661 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
      721 tgcctttaat aaaggcgaaa cagcgatgac c
//
