LOCUS Exported 9935 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLentiEKAR2G1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9935) AUTHORS Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O TITLE A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. JOURNAL Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. PUBMED 23882122 REFERENCE 2 (bases 1 to 9935) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9935 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 26..130 /gene="bla" /label=AmpR promoter CDS 131..991 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(349..368) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1162..1750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1651..1670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1904..1921 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2038..2059 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2074..2104 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2112..2128 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2117..2139 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2136..2152 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2136..2152 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2171..2191 /label=T3 /note="T3 promoter, forward primer" promoter 2173..2191 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2447..2627 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2671..2796 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3289..3522 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3707..3751 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4012..4129 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind complement(4135..4155) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4140..4469 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4320..4455 /label=SV40 ori /note="SV40 origin of replication" CDS 4479..5078 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(4479..4498) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4975..4995 /label=Puro-F /note="Puromycin resistance gene, forward primer" enhancer 5150..5453 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5454..5657 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5608..5628 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 5654..5678 /label=LNCX /note="Human CMV promoter, forward primer" CDS 5757..5774 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 5784..5813 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 7134..7868 /codon_start=1 /product="circularly permuted form of the Venus yellow fluorescent protein (Nagai et al., 2004)" /label=cp173Venus /note="efficient FRET acceptor for CFP" /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN VYITADKQKNGIKANFKIRHNIE" primer_bind 7271..7292 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(7395..7416) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7656..7675) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" CDS 7938..7955 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 7997..8585 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8050..8070) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8468..8479) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8676..8909 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8981..9102 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9018..9037) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9072..9091 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9121..9256 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(9288..9307) /label=T7 /note="T7 promoter, forward primer" promoter complement(9289..9307) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9317..9334) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9317..9333) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9326..9348) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9478..9933 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9565..9584) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9775..9796 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg 2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 2401 cattgcagag atattgtatt taagtgccta gctcgataca ataaacgggt ctctctggtt 2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac 2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa 2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc 2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga 2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat 2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca 2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa 3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag 3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc 3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg 3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac 3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt 3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt 3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat 3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag 3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc 3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct 3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttgtggaatg 4141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 4201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 4261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 4321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 4381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 4441 gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg 4501 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 4561 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 4621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 4681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 4741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 4801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 4861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 4921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 4981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc 5041 gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc 5101 gcccgaccga aaggagcgca cgacccaatc gataagcttg ggagttccgc gttacataac 5161 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 5221 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 5281 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 5341 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 5401 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 5461 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 5521 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 5581 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 5641 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct 5701 gttttgacct ccatagaaga caccgactct agaggatccg gagatatacc atggcacacc 5761 atcaccacca ccacgggagc ggggagcaga aactaatatc agaggaggat cttggctcag 5821 gttcaggatc ctccaccgac ggcatggacg agctgtacaa gggtgctagc ggaggaatgg 5881 tgagcaaggg cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga 5941 agatggaggg caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc 6001 cctacgacgg caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct 6061 cctacgacat tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg 6121 acgacatccc caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca 6181 tgaccttcga ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact 6241 ccttcatcta cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc 6301 agaagaagac caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc 6361 tgaagggcga cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact 6421 tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc 6481 accgcatcga gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg 6541 ccgtggcccg caactccgga atggcggacg aggagaagct gccgcccggc tgggagaagc 6601 gcatgagccg cagctcaggc cgagtgtact acttcaacca catcactaac gccagccagt 6661 gggagcggcc cagcggcaac agcagcagtg gtggcaaaaa cgggcagggg gagcctgcca 6721 ggaagctgcg cattctgcag tcgactaggg agcaagggct tgtgcccggg tctagaggcg 6781 gaggtggtgg aggaggtgga ggtggtggag gtggaggtgg aggtggtggc ggtggtggag 6841 gaggaggagc tagaggcgga ggtggtggag gaggtggagg tggtggaggt ggaggtggag 6901 gtggtggcgg tggtggagga ggaggagcca gcggcggagg tggtggagga ggtggaggtg 6961 gtggaggtgg aggtggaggt ggtggcggtg gtggaggagg aggaaccggt gctgaccaac 7021 tgactgaaga gtggtcgacg gtacagcgga ccgcgcctga cgtaccacgt acaccagtag 7081 gaaaggcaaa gctttcattc caattcccgc gggcgcggga catcgcggcc gcgatggacg 7141 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 7201 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga 7261 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 7321 acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag ctgttcaccg 7381 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 7441 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca 7501 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgatgt 7561 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 7621 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 7681 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 7741 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 7801 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca 7861 acatcgaggg taccgatatc cttcaaaaaa aacttgaaga acttgaactt gacgaatgag 7921 atatctaatg actcgagcac caccatcacc atcaccagga tcctgcagtc tagagctagc 7981 atatgaattc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 8041 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 8101 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 8161 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 8221 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 8281 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 8341 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 8401 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 8461 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 8521 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 8581 cccgcctgga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt 8641 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 8701 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 8761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 8821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 8881 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 8941 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 9001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 9061 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 9121 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 9181 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 9241 ggcttttttg gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt 9301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 9361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 9421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg 9481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 9541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 9601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 9661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 9721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 9781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 9841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 9901 cgaattttaa caaaatatta acgtttacaa tttcc //