LOCUS Exported 8678 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Ubc-megf10-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8678) AUTHORS Kay JN, Chu MW, Sanes JR TITLE MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons. JOURNAL Nature. 2012 Mar 11;483(7390):465-9. doi: 10.1038/nature10877. PUBMED 22407321 REFERENCE 2 (bases 1 to 8678) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8678 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 46..1257 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 1094..1113 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" protein_bind 1325..1349 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(1356..1367) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind complement(4846..4870) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 4890..4899 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4896..5615 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4896..5612 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4941..4962) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5202..5221) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5549..5570 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(5702..5721) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 5767..5822 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5821..5840) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(6181..6197) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6181..6197) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6194..6216) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6205..6221 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6229..6259) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6274..6295 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6354..6549 /label=SV40 promoter /note="SV40 early promoter" rep_origin 6400..6535 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6462..6481 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 6555..6689 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6592..6611) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6646..6665 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6757..6774) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6928..7516) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7008..7027) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7687..8547) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8310..8329 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8548..8652) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agtaacccgt gtcggctcca gatctggcct ccgcgccggg 61 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag 121 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat 181 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg 241 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc 301 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg 361 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg 421 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt 481 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct 541 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc 601 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag 661 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa 721 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc 781 actgactgga gaactcgggt ttgtcgtctg gttgcggggg cggcagttat gcggtgccgt 841 tgggcagtgc acccgtacct ttgggagcgc gcgcctcgtc gtgtcgtgac gtcacccgtt 901 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc 961 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac 1021 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat 1081 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt 1141 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag 1201 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa 1261 gcttgggctg caggtcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggccca 1321 tcaaacaagt ttgtacaaaa aagcaggctc cgaattcgcc cttcgattgt tcttcacaga 1381 aaatggcgat ttcttcaagt tcgtgcctgg gcctcatctg ctcactgctc tgtcactggg 1441 tggggacagc atcctccctg aacctggaag accccaacgt atgcagccac tgggaaagct 1501 actcggtgac tgtgcaggag tcgtatccac atcccttcga tcagatctac tacacaagct 1561 gcaccgacat cctgaactgg tttaaatgca cacggcacag aatcagctac cggacagcct 1621 accgccacgg ggagaaaacc atgtatagac gcaaatccca gtgttgccca ggattttatg 1681 aaagccgaga catgtgtgtc cctcactgtg ctgataaatg tgtccatggt cgctgcattg 1741 ctccaaacac ctgtcagtgt gagcctggct ggggtgggac caactgtagc agtgcttgtg 1801 atggtgatca ctgggggcct cactgcagca gccgatgcca gtgcaaaaac agagctttgt 1861 gtaaccccat caccggtgct tgccactgcg ctgcgggcta ccggggatgg cgctgcgagg 1921 accgttgtga acagggcacg tacggtaacg actgtcacca aagatgccag tgtcagaatg 1981 gagcgacctg tgaccacatc actggggaat gccgttgttc acctgggtac actggagcct 2041 tctgtgagga tctttgtcct cctggcaaac atggtccaca ttgtgagcag aggtgtccct 2101 gccaaaatgg gggtgtgtgc caccatgtca ctggagagtg ctcttgccct tctggttgga 2161 tgggcacagt gtgtggtcag ccctgccctg agggtcgctt tggaaagaac tgttcccaag 2221 aatgccagtg tcacaatgga ggaacgtgtg atgctgccac aggccagtgt cactgcagcc 2281 caggatacac aggggaacgg tgtcaggacg aatgtcctgt tgggagctat ggagttcgct 2341 gtgctgaggc ctgcaggtgt gtcaacggag gcaagtgtta ccacgtgagt ggtacatgcc 2401 tgtgcgaagc aggcttttcg ggtgaacttt gcgaggcgcg cctgtgtccc gaggggcttt 2461 acggcatcaa atgtgacaag cggtgcccct gccacctgga caacactcac agctgtcatc 2521 ccatgtctgg agagtgtggc tgcaagccgg gttggtcggg actgtactgt aatgaaacat 2581 gctcccctgg attctacggg gaggcttgcc aacagatctg cagctgccag aacggggcgg 2641 actgcgacag tgtgactgga aggtgtgcct gcgctccagg attcaaaggg actgactgct 2701 ctactccgtg tcctctggga cgctacggga taaattgttc ttctcgctgt ggctgtaaaa 2761 atgatgctgt ctgttctcct gtggatggat catgtatctg taaggcaggc tggcacgggg 2821 tggactgttc catccgctgc cccagtggca catggggctt tggctgtaac ctaacgtgtc 2881 agtgcctcaa tggcggtgcc tgcaacacgc tggatgggac ctgcacctgt gcgcccggat 2941 ggcgaggcgc gaagtgtgaa tttccctgcc aggatggcac ttatgggctg aactgtgccg 3001 agcgctgtga ctgcagccat gcagatggct gtcaccccac tacaggccat tgccgctgcc 3061 tccctggatg gtcaggtgtg cactgtgaca gtgtgtgcgc tgagggacgc tggggtccta 3121 actgctcgct gccctgctac tgtaaaaatg gggcttcgtg ttctccggat gatggcatct 3181 gtgagtgtgc acccggattc cgaggcacca cttgccagag aatctgctcc cccggttttt 3241 atggacatcg ctgtagccag acctgcccgc agtgtgtgca cagcagtggg ccctgccacc 3301 acatcacggg cctgtgtgac tgcttacctg gcttcaccgg tgccctgtgc aatgaagtgt 3361 gtcccagtgg cagatttggg aaaaactgtg caggcgtttg tacttgcacc aacaatggca 3421 cctgtaaccc catcgacaga tcctgccagt gttacccagg ctggattggc agtgactgct 3481 cccagccctg tccacctgcg cactggggtc cgaactgcat ccacacctgc aactgccaca 3541 acggagcctt ttgcagcgcc tatgatgggg aatgcaaatg cactcctggc tggacggggc 3601 tctactgcac tcagagatgc cctctgggct tctatggtaa ggactgtgca ctgatatgcc 3661 aatgtcaaaa cggagctgac tgcgaccata tctcggggca gtgtacctgc cgcacgggat 3721 tcatgggacg gcactgtgaa cagaagtgcc ctgcgggaac atacggctat ggctgtcgcc 3781 agatctgtga ctgtctgaac aactccacct gtgaccacat cactggcacg tgttactgta 3841 gcccaggatg gaaaggggca cgatgtgacc aagctggggt tatcatcgtg ggcaatctga 3901 acagcttaag ccggaccagc accgcccttc ctgccgattc ctatcagatc ggggccatcg 3961 cgggcatcgt ggtcctcgtt cttgttgtgc tcttcctgct ggcgctgttc atcatctaca 4021 gacacaagca gaagaggaag gaatcaagca tgccggccgt gacctacacc cccgccatga 4081 gggtcatcaa tgcagactat accatcgcag aaaccctgcc tcacagcaat ggtggaaatg 4141 ccaacagcca ctactttacc aatcccagtt atcacacact tagccagtgt gccacatccc 4201 ctcatgtgaa caatagggac aggatgacca ttgcaaagtc aaaaaacaat cagctgtttg 4261 tgaatcttaa aaatgtgaat ccagggaaga gagggacatt ggtggactgc actgggacat 4321 tgccagctga ctggaagcaa ggaggctacc tcaatgagct tggtgctttc gggctggaca 4381 gaagctacat gggaaagtcc ttaaaagatc tggggaagaa ctctgaatat aattcaagca 4441 cttgctcctt aagcagctct gaaaacccat atgccaccat taaagacccg cctgcactcc 4501 tgcctaaaag ctccgagtgc ggctacgtgg agatgaagtc gccggcgcgg agagactccc 4561 catatgcaga gatcaacaac tcaactccag ccaacaggaa tgtctatgaa gtcgaaccta 4621 cagtgagcgt tgtgcaagga gtattcagca acagcggtca cgtcacccaa gacccatatg 4681 accttccaaa gaacagtcac atcccttgcc attatgacct gctgccagta agggacagtt 4741 catcctcccc aaagagagag gatggtggtg gcagcaacag caccagcagc aacagcacca 4801 gcagcagcag cagcagcagt gaatcacatc aaaagggcga attcgaccca gctttcttgt 4861 acaaagtggt tcgatgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt 4921 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 4981 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 5041 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 5101 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 5161 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 5221 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 5281 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 5341 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 5401 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 5461 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 5521 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 5581 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcactcct caggtgcagg 5641 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag 5701 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt 5761 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct 5821 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt 5881 agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt 5941 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact 6001 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa 6061 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata 6121 gctgtccctc ttctcttatg aagatccctc gacctgcagc ccaagcttgg cgtaatcatg 6181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 6301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc 6361 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 6421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 6541 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 6601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6661 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg 6721 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 6781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6901 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6961 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7021 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7081 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 7141 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 7201 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 7261 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 7321 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 7381 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 7441 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 7501 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 7561 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7621 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7681 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7741 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7801 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7861 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7921 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7981 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8041 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 8101 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 8161 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 8221 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 8281 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 8341 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 8401 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 8461 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 8521 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 8581 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8641 aggggttccg cgcacatttc cccgaaaagt gccacctg //