LOCUS Exported 870 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION Bacterial expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS ACACA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 870) TITLE SGC+year JOURNAL unpublished REFERENCE 2 (bases 1 to 870) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..870 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 11..33 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 44..61 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 86..106 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" ORIGIN 1 tctagaatat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca 61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatcg gcttgcacct 121 ggttaagcag ggtcgcgacc gtaagaaaat cgattctcaa cgcgacttta ctgtcgcgtc 181 accagcagag ttcgtaacac gttttggcgg taataaggtg atcgagaaag ttttgattgc 241 caacaatggc atcgctgcgg tcaagtgtat gcgcagtatt cgtcgctggt cgtacgaaat 301 gttccgtaac gagcgcgcca tccgtttcgt ggtaatggtg acccctgagg acctcaaggc 361 aaacgctgag tatattaaga tggccgacca ttacgttccg gtccccggcg gcccaaataa 421 caataactat gcgaacgtcg agctgatctt ggatattgca aagcgcatcc ctgtgcaggc 481 tgtttgggcc ggttggggcc acgcgagcga gaatccgaaa ctgcccgaac tcctcctcaa 541 gaacggtatc gcttttatgg gcccaccttc ccaggccatg tgggcactgg gtgacaaaat 601 tgcatcttca atcgtcgctc aaactgccgg cattccgaca ctgccctgga gtggtagcgg 661 cttgcgtgtg gattggcagg aaaatgactt ctccaaacgc atcctgaacg taccccaaga 721 gttgtacgaa aagggttatg ttaaagatgt cgacgatggc ctgcaggcag ccgaggaagt 781 gggttatccc gtaatgatta aggcgtcgga gggcggcggc ggtaaaggca tccgtaaagt 841 aaacaatgct gacgattttc cgaacctgtt //