LOCUS       Exported                 827 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMJ823
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E
  TITLE     A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive 
            Bacterial Immunity.
  JOURNAL   Science. 2012 Jun 28.
  PUBMED    22745249
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             28..354
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             376..393
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             406..426
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttgtttact ttagaaggag atataccatg agcgataaaa ttattcacct gactgacgac
       61 agttttgaca cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag
      121 tggtgcggtc cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag
      181 ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat
      241 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
      301 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccggatct
      361 ggcagtggtt ctggtcatca ccatcaccat cactccgcgg gtagcgagaa tctttatttt
      421 cagggcgcca tggaaaaacc gtacacgatt ggactagaca taggaacaaa ctcggttgga
      481 tgggcagtgt taacagatca atatgattta gtgaagagga aaatgaaaat tgctggagac
      541 tctgagaaaa aacagataaa gaaaaatttc tggggagtta gactatttga tgagggtcaa
      601 acggcagctg atagaagaat gactagaaca gctagaagga gaatagagag aagacgcaat
      661 cgtatttcgt atttacaagg gatatttgcg gaagaaatgt ctaaggtgga tgcaaatttc
      721 ttttgccgat taagtgatag cttttatgtt gataacgaaa aaagaaatag tcgtcatcct
      781 ttttttgcga ctatagagga agaagtagaa taccacaaaa actaccc
//
